A2909
兔IgG-琼脂糖
saline suspension
别名:
IgG琼脂糖珠
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Conjugate:
agarose conjugate
Technique(s):
immunoprecipitation (IP): suitable
Application:
immunoprecipitation (IP)
Citations:
49
Application
兔IgG-琼脂糖已被用于免疫沉淀 和亲和纯化检测。
将与琼脂糖珠交联的兔IgG抗体（20 μl/ml）用于从293T细胞的全细胞提取物中分离RAT标记的蛋白。将交联至琼脂糖珠的兔IgG用于从哺乳动物全细胞提取物中纯化标记蛋白质（0.5至1.0mg总蛋白质结合至10 μl珠子上）。
Disclaimer
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General description
IgG是调节几种免疫应答的糖蛋白抗体。IgG-琼脂糖是一种免疫吸附剂，可用于纯化抗体，去除物种特异性交叉反应抗体，或从抗血清制剂中去除污染抗体。一般来说，可以使用相等树脂体积的IgG-琼脂糖从抗血清制剂中除去交叉反应抗体。然而，树脂与抗血清的比例将随个体应用而变化。免疫球蛋白G（IgG）是免疫球蛋白家族的一部分，并且是一种广泛表达的血清抗体。它的恒定（C）区域包含一条γ 重链。IgG的单体150kDa结构分别由两条相同的重链和两条相同的轻链组成，其分子量分别为50kDa和25kDa。该抗体的一级结构还含有二硫键，可用于连接两条重链、连接重链和轻链、以及链内的连接。IgG可进一步细分为四类，即具有不同重链（γ 1、γ 2、γ 3和γ 4）的IgG1、IgG2、IgG3和IgG4。
Other Notes
Physical form
其悬浮于含有防腐剂的0.5M NaCl中。
存储类别
10 - Combustible liquids
wgk
WGK 3
法规信息
低风险生物材料
此项目有
A Colley et al.
Molecular and cellular biology, 20(19), 7238-7246 (2000-09-13)
Putative RNA helicases are involved in most aspects of gene expression. All previously characterized members of the DEAH-box family of putative RNA helicases are involved in pre-mRNA splicing. Here we report the analysis of two novel DEAH-box RNA helicases, Dhr1p
Emmanuel Vanrobays et al.
RNA (New York, N.Y.), 14(10), 2061-2073 (2008-08-30)
Eukaryotic ribosome synthesis is a highly dynamic process that involves the transient association of scores of trans-acting factors to nascent pre-ribosomes. Many ribosome synthesis factors are nucleocytoplasmic shuttling proteins that engage the assembly pathway at early nucleolar stages and escort
Lingjuan Shan et al.
Journal of genetics and genomics = Yi chuan xue bao, 44(2), 95-106 (2017-02-14)
In the sexually reproductive organisms, gametes are produced by meiosis following a limited mitotic amplification. However, the intrinsic program switching cells from mitotic to meiotic cycle is unclear. Alternative polyadenylation (APA) is a highly conserved means of gene regulation and
Xiaowen Wang et al.
Nature, 524(7566), 481-484 (2015-07-21)
Mitochondria are multifunctional organelles whose dysfunction leads to neuromuscular degeneration and ageing. The multi-functionality poses a great challenge for understanding the mechanisms by which mitochondrial dysfunction causes specific pathologies. Among the leading mitochondrial mediators of cell death are energy depletion
Lionel Tafforeau et al.
The EMBO journal, 25(19), 4547-4556 (2006-10-04)
We describe a new member of the F-box family, Pof14, which forms a canonical, F-box dependent SCF (Skp1, Cullin, F-box protein) ubiquitin ligase complex. The Pof14 protein has intrinsic instability that is abolished by inactivation of its Skp1 interaction motif
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