一般描述
IgG是调节几种免疫应答的糖蛋白抗体。IgG-琼脂糖是一种免疫吸附剂，可用于纯化抗体，去除物种特异性交叉反应抗体，或从抗血清制剂中去除污染抗体。一般来说，可以使用相等树脂体积的IgG-琼脂糖从抗血清制剂中除去交叉反应抗体。然而，树脂与抗血清的比例将随个体应用而变化。免疫球蛋白G（IgG）是免疫球蛋白家族的一部分，并且是一种广泛表达的血清抗体。它的恒定（C）区域包含一条γ 重链。IgG的单体150kDa结构分别由两条相同的重链和两条相同的轻链组成，其分子量分别为50kDa和25kDa。该抗体的一级结构还含有二硫键，可用于连接两条重链、连接重链和轻链、以及链内的连接。IgG可进一步细分为四类，即具有不同重链（γ 1、γ 2、γ 3和γ 4）的IgG1、IgG2、IgG3和IgG4。
应用
兔IgG-琼脂糖已被用于免疫沉淀 和亲和纯化检测。
将与琼脂糖珠交联的兔IgG抗体（20 μl/ml）用于从293T细胞的全细胞提取物中分离RAT标记的蛋白。将交联至琼脂糖珠的兔IgG用于从哺乳动物全细胞提取物中纯化标记蛋白质（0.5至1.0mg总蛋白质结合至10 μl珠子上）。
外形
其悬浮于含有防腐剂的0.5M NaCl中。
其他说明
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储存分类代码
10 - Combustible liquids
WGK
WGK 3
法规信息
低风险生物材料
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