一般描述
二硝基苯(DNP)是一种半抗原,用于在免疫分析中标记一级或二级探针。因此,标记可检测底物的DNP抗体是研究靶蛋白的实用工具。小鼠抗-DNP-碱性磷酸酶标记抗体对DNP具有特异性。
免疫原
DNP-KLH偶联物
应用
小鼠抗-DNP-碱性磷酸酶标记抗体可用于ELISA (1:25,000)和斑点印迹(1:180,000)分析。
该抗体可用于受控过敏反应的实验诱导或作为小鼠IgE来源。
该抗体可用于受控过敏反应的实验诱导或作为小鼠IgE来源。为了诱导全身性过敏反应,给小鼠注射3 μg抗DNP小鼠IgE,20小时后静脉注射500 μg DNP-BSA。为了诱导局部过敏反应,给小鼠皮内注射10ng抗DNP小鼠IgE,然后静脉注射500 μg DNP-BSA激发过敏反应。
外形
0.05 M Tris缓冲溶液,pH 8.0,含1 mM MgCl2、1% BSA、50%甘油和15 mM叠氮化钠
分析说明
抗-DNP单克隆抗体经检验对牛血清白蛋白的反应性极低。
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WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
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