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Merck
CN

A2831

Sigma-Aldrich

小鼠抗-DNP−碱性磷酸酶偶联物 小鼠抗

clone SPE-7, purified immunoglobulin, buffered aqueous glycerol solution

别名:

抗二硝基苯, 抗DNP单克隆抗体

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About This Item

UNSPSC代码:
12352203
NACRES:
NA.46

生物来源

mouse

质量水平

偶联物

alkaline phosphatase conjugate

抗体形式

purified immunoglobulin

抗体产品类型

primary antibodies

克隆

SPE-7, monoclonal

形式

buffered aqueous glycerol solution

技术

direct ELISA: 1:25,000 using DNP-BSA
dot blot: 1:180,000 (chemiluminescence)

同位素/亚型

IgE

运输

wet ice

储存温度

2-8°C

靶向翻译后修饰

unmodified

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一般描述

二硝基苯(DNP)是一种半抗原,用于在免疫分析中标记一级或二级探针。因此,标记可检测底物的DNP抗体是研究靶蛋白的实用工具。小鼠抗-DNP-碱性磷酸酶标记抗体对DNP具有特异性。

免疫原

DNP-KLH偶联物

应用

小鼠抗-DNP-碱性磷酸酶标记抗体可用于ELISA (1:25,000)和斑点印迹(1:180,000)分析。
该抗体可用于受控过敏反应的实验诱导或作为小鼠IgE来源。
该抗体可用于受控过敏反应的实验诱导或作为小鼠IgE来源。为了诱导全身性过敏反应,给小鼠注射3 μg抗DNP小鼠IgE,20小时后静脉注射500 μg DNP-BSA。为了诱导局部过敏反应,给小鼠皮内注射10ng抗DNP小鼠IgE,然后静脉注射500 μg DNP-BSA激发过敏反应。

外形

0.05 M Tris缓冲溶液,pH 8.0,含1 mM MgCl2、1% BSA、50%甘油和15 mM叠氮化钠

分析说明

抗-DNP单克隆抗体经检验对牛血清白蛋白的反应性极低。

免责声明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

常规特殊物品
含少量动物源组分生物产品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

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