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Merck
CN

A277

Sigma-Aldrich

Monoclonal Anti-Na+/K+ ATPase (α1 Subunit) antibody produced in mouse

clone M8-P1-A3, ascites fluid

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About This Item

MDL编号:
UNSPSC代码:
12352203

生物来源

mouse

偶联物

unconjugated

抗体形式

ascites fluid

抗体产品类型

primary antibodies

克隆

M8-P1-A3, monoclonal

分子量

antigen ~110 kDa

种属反应性

sheep, canine, rat, pig

请勿与下列物质发生反应

chicken, Xenopus

技术

immunohistochemistry (frozen sections): 1:100
western blot: 1:250

同位素/亚型

IgG1

UniProt登记号

运输

dry ice

储存温度

−20°C

基因信息

一般描述

Epitope mapping studies indicates the epitope is between amino acids 496 and 506 of lamb kidney Na+/K+ ATPase. No reactivity is observed in chicken or Xenopus. Staining is consistent with the plasma membrane localization of Na+/K+ ATPase.
Mouse monoclonal anti-Na+/K+ ATPase (α1 subunit) reacts with the α1 subunit of Na+/K+-ATPase in sheep, canine, rat and pig. In rat, the antibody shows no reactivity with the α2 or α3 subunits.
Na+/K+ transporting ATPase subunit α 1 is an ion transport pump critical for maintaining the gradient of Na and K ions across the plasma membrane.

免疫原

lamb kidney α1 subunit of Na+/K+ ATPase.

应用

Mouse monoclonal anti-Na+/K+ ATPase (α1 subunit) has been used for western blot and co-immunoprecipitation assays. The product can also be used for immunohistochemistry applications using frozen sections at a dilution of 1:100.

外形

Solution in phosphate buffered saline containing 0.05% sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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储存分类代码

10 - Combustible liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

新产品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Helen X Pu et al.
The Journal of biological chemistry, 277(23), 20270-20276 (2002-04-04)
The two variants of the gamma subunit of the rat renal sodium pump, gamma(a) and gamma(b), have similar effects on the Na,K-ATPase. Both increase the affinity for ATP due to a shift in the enzyme's E(1) <--> E(2) conformational equilibrium
Valdilene S Ribeiro et al.
Biochimica et biophysica acta, 1864(7), 2458-2471 (2018-04-15)
α-Tocopherol (α-Toc) overload increases the risk of dying in humans (E.R. Miller III et al. Meta-analysis: high-dosage vitamin E supplementation may increase all-cause mortality Ann Int Med. 142 (2005) 37-46), and overload during early development leads to elevation of blood
Zinaeli López-González et al.
Journal of molecular histology, 51(6), 701-716 (2020-10-19)
The kidney controls body fluids, electrolyte and acid-base balance. Previously, we demonstrated that hyperpolarization-activated and cyclic nucleotide-gated (HCN) cation channels participate in ammonium excretion in the rat kidney. Since acid-base balance is closely linked to potassium metabolism, in the present
Douglas R Yingst et al.
American journal of physiology. Renal physiology, 297(5), F1324-F1329 (2009-09-04)
To understand how rapid changes in blood pressure can regulate Na-K-ATPase in the kidney cortex, we tested the hypothesis that a short-term (5 min) decrease in renal perfusion pressure will increase the amount of Na-K-ATPase in the plasma membranes by
W J Ball et al.
Biochimica et biophysica acta, 719(3), 413-423 (1982-12-17)
Four stable hybridoma cell lines secreting antibodies specific to the membrane (Na+ + K+)-dependent ATPase isolated from lamb kidney medulla have been produced by fusing mouse myeloma cells with spleen cells from immunized mice. These cell lines produce IgG gamma

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