product name
偶氮酪蛋白, protease substrate
生物来源
bovine
质量水平
形式
powder
技术
ligand binding assay: suitable
溶解性
water: 5 mg/mL, clear, orange to very deep orange
ε (消光系数)
≥25 at 440 nm in 0.1 M NaOH at 1%
储存温度
2-8°C
一般描述
偶氮酪蛋白是酪蛋白的显色衍生物。偶氮酪蛋白经蛋白酶降解后产生可溶于TCA、具有高紫外线吸收率的偶氮肽。这一偶氮酪蛋白测定法广泛用于评估细菌在葡萄糖和无机盐合成底物上细菌发酵产生的蛋白酶量。
应用
偶氮酪蛋白已用作测定蛋白酶活性的底物。
偶氮酪蛋白是一种炎症因子，可用于在实验动物中诱导淀粉样蛋白A淀粉样变性。
偶氮酪蛋白是一种非特异性蛋白酶底物。 酪蛋白的水解将偶氮染料释放至培养基中，其可通过培养基在440nm处的吸光度进行检测。
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
动植物源性产品
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Journal of bacteriology, 191(24), 7509-7519 (2009-10-20)
The opportunistic human bacterial pathogen Pseudomonas aeruginosa produces membrane vesicles (MVs) in its surrounding environment. Several features of the P. aeruginosa MV production mechanism are still unknown. We previously observed that depletion of Opr86, which has a role in outer
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Leishmaniasis is a neglected tropical disease with diverse outcomes ranging from self-healing lesions, to progressive non-healing lesions, to metastatic spread and destruction of mucous membranes. Although resolution of cutaneous leishmaniasis is a classic example of type-1 immunity leading to self-healing
Nicolas Oswaldo Gomez et al.
Molecular microbiology, 115(1), 84-98 (2020-09-09)
To overcome the metal restriction imposed by the host's nutritional immunity, pathogenic bacteria use high metal affinity molecules called metallophores. Metallophore-mediated metal uptake pathways necessitate complex cycles of synthesis, secretion, and recovery of the metallophore across the bacterial envelope. We
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