跳转至内容
Merck
CN
所有图片(2)

文件

A25120

Sigma-Aldrich

DEAE–Sephadex® A-25 氯化物形式

别名:

DEAE Sephadex®, 二乙基氨基乙基葡聚糖®

登录查看公司和协议定价
所有图片(2)

About This Item

CAS号:
12609-80-2
MDL编号:
MFCD00132778
UNSPSC代码:
47101511
NACRES:
NA.56

形式

powder

质量水平

200

技术

activity assay: suitable

基质

cross-linked dextran

基质活性基团

functional group

珠子尺寸

40-125 μm (dry)

孔径

~30,000 Da exclusion limit

工作pH值

2-9

容量

3-4 meq/g ion exchange capacity

相容性

mode of use mixed weak and strong anion exchange chromatography

一般描述

Sephadex® 离子交换剂含有具有官能团的交联葡聚糖基质。这些基团通过稳定的醚键连接到基质中的葡萄糖单元。DEAE Sephadex 可用于离子交换色谱法纯化和分离蛋白质。

A25120-100G′s 更新货号为 GE17-0170-01
A25120-500G′s 更新货号为 GE170180-02

应用

DEAE® Sephadex A-25 已用于：
  • 形成 DEAE Sephadex A-25（甲酸盐形式），用于使用辣根植物提取硫代葡萄糖苷（GLS）
  • 甘蓝芽中 GLS 含量的测定
  • 星形胶质细胞制备和纯化恩多泽平
  • 在急性心肌梗死（AMI）患者中鉴定和标记胰岛素激活的一氧化氮合酶抑制剂（蛋白）

特点和优势

  • 弱阴离子交换剂
  • 适用于批处理技术
  • 高结合能力
  • 对常用的水性缓冲剂添加剂（如 8M 尿素和 6M 盐酸胍）无反应

法律信息

DEAE Sephadex is a registered trademark of Cytiva
Sephadex is a registered trademark of Cytiva

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How should I store Product A25120, DEAE-Sephadex® A-25 chloride?

    Unused media can be stored in the container at +4 to +30 °C.  Ensure that the screw-top is fully tightened.  Packed columns should be equilibrated in working buffer containing 20% ethanol to prevent microbial growth.

  4. What is the chemical stability of Product A25120, DEAE-Sephadex® A-25 chloride form? 

    According to the manufacturer, this is compatible with all commonly used aqueous buffers, 1 M NaOH, 8 M urea, 6 M guanidine hydrochloride and 70% ethanol.

  5. How do I equilibrate Product A25120, DEAE-Sephadex® A-25 chloride form?

    After packing, and before a chromatographic run, equilibrate with working buffer by washing with at least 5 bed volumes.

  6. How should I regenerate Product A25120, DEAE-Sephadex® A-25 chloride form?

    After each separation, elute any reversibly bound material either with a high ionic strength solution (e.g. 1 M NaCl in buffer) or by increasing pH. Regenerate the media by washing with at least 5 bed volumes of buffer, or until the column effluent shows stable conductivity and pH values.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Shu-Kui Wang et al.
Zhonghua nan ke xue = National journal of andrology, 8(3), 173-177 (2002-12-14)
To establish and evaluate the anti-human seminal plasma phospholipase A2 (PLA2) monoclonal antibody (McAb). After having been separated and purified from human seminal plasma by PEG precipitation, Sephacryl S-300 column chromatography, DEAE-Sephadex A-25 column chromatography and HA column chromatography, PLA2
DEAE-silica gel and DEAE-controlled porous glass as ion exchangers for the isolation of glycolipids.
S K Kundu
Methods in enzymology, 72, 174-185 (1981-01-01)
M J Hollingsworth et al.
Molecular and cellular biology, 6(4), 1058-1064 (1986-04-01)
A requisite step in the biosynthesis of tRNA is the removal of 5' leader sequences from tRNA precursors. We have detected an RNase P activity in yeast mitochondrial extracts that can carry out this reaction on a homologous precursor tRNA.
Scott N Boyle et al.
Biochemistry, 46(41), 11614-11620 (2007-09-26)
Abl family kinases have been implicated in the regulation of cell morphogenesis and migration, but the molecular mechanisms through which they operate are not fully elucidated. We applied the bump-hole technique, pioneered by Shokat and colleagues, to identify direct substrates
Dong-Lin Wang et al.
Journal of agricultural and food chemistry, 50(2), 262-265 (2002-01-10)
Among 10 lipases tested, Candida rugosa lipase exhibited the best ability to catalyze the resolution of dl-menthol in organic solvent. The lipase was immobilized on different carriers, and the experiment was carried out with different acyl donors. The high yield
查看所有相关文献

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门