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Merck
CN

A25120

DEAE–Sephadex® A-25 氯化物形式

别名:

DEAE Sephadex®, 二乙基氨基乙基葡聚糖®

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化学文摘社编号:
12609-80-2
UNSPSC Code:
47101511
NACRES:
NA.56
MDL number:
MFCD00132778

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form

powder

technique(s)

activity assay: suitable

matrix

cross-linked dextran

matrix active group

functional group

bead size

40-125 μm (dry)

pore size

~30,000 Da exclusion limit

operating pH

2-9

capacity

3-4 meq/g ion exchange capacity

compatibility

mode of use mixed weak and strong anion exchange chromatography

Quality Level

200

Application

DEAE® Sephadex A-25 已用于：
  • 形成 DEAE Sephadex A-25（甲酸盐形式），用于使用辣根植物提取硫代葡萄糖苷（GLS）
  • 甘蓝芽中 GLS 含量的测定
  • 星形胶质细胞制备和纯化恩多泽平
  • 在急性心肌梗死（AMI）患者中鉴定和标记胰岛素激活的一氧化氮合酶抑制剂（蛋白）

Features and Benefits

  • 弱阴离子交换剂
  • 适用于批处理技术
  • 高结合能力
  • 对常用的水性缓冲剂添加剂（如 8M 尿素和 6M 盐酸胍）无反应

General description

Sephadex® 离子交换剂含有具有官能团的交联葡聚糖基质。这些基团通过稳定的醚键连接到基质中的葡萄糖单元。DEAE Sephadex 可用于离子交换色谱法纯化和分离蛋白质。

A25120-100G′s 更新货号为 GE17-0170-01
A25120-500G′s 更新货号为 GE170180-02

Legal Information

DEAE Sephadex is a registered trademark of Cytiva
Sephadex is a registered trademark of Cytiva

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000514869
0000514868
0000514868
0000514869
0000486762

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How should I store Product A25120, DEAE-Sephadex® A-25 chloride?

    Unused media can be stored in the container at +4 to +30 °C.  Ensure that the screw-top is fully tightened.  Packed columns should be equilibrated in working buffer containing 20% ethanol to prevent microbial growth.

  4. What is the chemical stability of Product A25120, DEAE-Sephadex® A-25 chloride form? 

    According to the manufacturer, this is compatible with all commonly used aqueous buffers, 1 M NaOH, 8 M urea, 6 M guanidine hydrochloride and 70% ethanol.

  5. How do I equilibrate Product A25120, DEAE-Sephadex® A-25 chloride form?

    After packing, and before a chromatographic run, equilibrate with working buffer by washing with at least 5 bed volumes.

  6. How should I regenerate Product A25120, DEAE-Sephadex® A-25 chloride form?

    After each separation, elute any reversibly bound material either with a high ionic strength solution (e.g. 1 M NaCl in buffer) or by increasing pH. Regenerate the media by washing with at least 5 bed volumes of buffer, or until the column effluent shows stable conductivity and pH values.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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The greatly enhanced fluorescence of Tb3+ when complexed with dipicolinic acid affords a simple and highly sensitive method for monitoring continuous anion flux through the erythrocyte anion transporter, band 3. Dipicolinic acid (pyridine-2,6-dicarboxylic acid) is entrapped in human erythrocytes and
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