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一般描述
酒精氧化酶（AOX）是一种均八聚物，分子量为600-kDa。它具有八个黄素腺嘌呤二核苷酸（FAD）辅因子。它属于葡萄糖-甲醇-胆碱（GMC）氧化还原酶家族。
应用
毕赤酵母来源酒精氧化酶溶液用于棉质果胶甲基酯酶（PME）的酶测定中。它也可以可视化的生物传感器形式固定在聚苯胺（PANI）膜上，以试纸的形式用于测定发酵饮料样品中的乙醇。
乙醇氧化酶可用于研究蛋白向过氧化物酶体的转位。产品 A2404 来自 毕赤酵母 。已用于 Hv-p68 cDNA 克隆的细菌表达和免疫学验证 。
生化/生理作用
醇氧化酶催化短链初级脂肪醇氧化成各自的醛。对甲醇的亲和力最高。醇氧化酶参与甲醇的代谢，这对利用甲醇作为唯一能源的酵母菌来说很重要。乙醇氧化酶主要存在于过氧化物酶体，但也可能存在于细胞质中。
单位定义
一个单位将在 pH 7.5 下在 25°C 下每分钟将 1.0μmole 甲醇氧化成甲醛。
外形
30% 蔗糖溶液，含 0.1 M 磷酸盐缓冲液 (pH 8.0)
储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Christina Dorado et al.
Biology, 10(12) (2021-12-25)
Florida citrus production has declined 75% due to Huanglongbing (HLB), a disease caused by the pathogenic bacterium Candidatus Liberibacter asiaticus (CLas). Methods to combat CLas are costly and only partially effective. The cross-compatible species Poncirus trifoliata and some of its
A simple visual ethanol biosensor based on alcohol oxidase immobilized onto polyaniline film for halal verification of fermented beverage samples
Kuswandi B, et al.
Sensors, 14(2), 2135-2149 (2014)
A I Soldevila et al.
The Journal of biological chemistry, 276(7), 4652-4661 (2000-11-01)
We have cloned and sequenced a novel alcohol oxidase (Hv-p68) from the filamentous fungus Helminthosporium (Cochliobolus) victoriae that copurifies with mycoviral double-stranded RNAs. Sequence analysis revealed that Hv-p68 belongs to the large family of FAD-dependent glucose methanol choline oxidoreductases and
Structure of alcohol oxidase from Pichia pastoris by cryo-electron microscopy
Vonck J, et al.
PLoS ONE, 11(7), e0159476-e0159476 (2016)
Henry Temple et al.
Nature plants, 8(6), 656-669 (2022-06-11)
Polysaccharide methylation, especially that of pectin, is a common and important feature of land plant cell walls. Polysaccharide methylation takes place in the Golgi apparatus and therefore relies on the import of S-adenosyl methionine (SAM) from the cytosol into the
实验方案
To measure alcohol oxidase activity, this assay uses 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) and a continuous spectrophotometric rate determination at 405 nm.
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