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一般描述
酒精氧化酶（AOX）是一种均八聚物，分子量为600-kDa。它具有八个黄素腺嘌呤二核苷酸（FAD）辅因子。它属于葡萄糖-甲醇-胆碱（GMC）氧化还原酶家族。
应用
毕赤酵母来源酒精氧化酶溶液用于棉质果胶甲基酯酶（PME）的酶测定中。它也可以可视化的生物传感器形式固定在聚苯胺（PANI）膜上，以试纸的形式用于测定发酵饮料样品中的乙醇。
乙醇氧化酶可用于研究蛋白向过氧化物酶体的转位。产品 A2404 来自 毕赤酵母 。已用于 Hv-p68 cDNA 克隆的细菌表达和免疫学验证 。
生化/生理作用
醇氧化酶催化短链初级脂肪醇氧化成各自的醛。对甲醇的亲和力最高。醇氧化酶参与甲醇的代谢，这对利用甲醇作为唯一能源的酵母菌来说很重要。乙醇氧化酶主要存在于过氧化物酶体，但也可能存在于细胞质中。
单位定义
一个单位将在 pH 7.5 下在 25°C 下每分钟将 1.0μmole 甲醇氧化成甲醛。
外形
30% 蔗糖溶液，含 0.1 M 磷酸盐缓冲液 (pH 8.0)
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
Pectin methylesterase and pectin remodelling differ in the fibre walls of two gossypium species with very different fibre properties
Liu Q, et al.
PLoS ONE, 8(6), e65131-e65131 (2013)
Xuan Wang et al.
Journal of experimental botany, 71(9), 2629-2640 (2020-02-02)
De-esterification of homogalacturonan (HG) is thought to stiffen pectin gels and primary cell walls by increasing calcium cross-linking between HG chains. Contrary to this idea, recent studies found that HG de-esterification correlated with reduced stiffness of living tissues, measured by
Christina Dorado et al.
Biology, 10(12) (2021-12-25)
Florida citrus production has declined 75% due to Huanglongbing (HLB), a disease caused by the pathogenic bacterium Candidatus Liberibacter asiaticus (CLas). Methods to combat CLas are costly and only partially effective. The cross-compatible species Poncirus trifoliata and some of its
Turn air-captured CO2 with methanol into amino acid and pyruvate in an ATP/NAD(P)H-free chemoenzymatic system.
Liu, et al.
Nature Communications, 14, 2772-2772 (2023)
A I Soldevila et al.
The Journal of biological chemistry, 276(7), 4652-4661 (2000-11-01)
We have cloned and sequenced a novel alcohol oxidase (Hv-p68) from the filamentous fungus Helminthosporium (Cochliobolus) victoriae that copurifies with mycoviral double-stranded RNAs. Sequence analysis revealed that Hv-p68 belongs to the large family of FAD-dependent glucose methanol choline oxidoreductases and
实验方案
To measure alcohol oxidase activity, this assay uses 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) and a continuous spectrophotometric rate determination at 405 nm.
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