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Merck
CN

A2050

Sigma-Aldrich

抗生物素蛋白-FITC 来源于鸡蛋白

buffered aqueous solution

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别名:
Avidin–Fluorescein isothiocyanate
MDL编号:
UNSPSC代码:
12352203
NACRES:
NA.46

偶联物

FITC conjugate

质量水平

形式

buffered aqueous solution

标记范围

3-5.5 mol fluorochrome per mol protein

技术

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 20-40 μg/mL
indirect immunofluorescence: 1:200

UniProt登记号

运输

dry ice

储存温度

−20°C

基因信息

chicken ... AVD(396260)

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一般描述

抗生物素蛋白是从蛋清中获得的同源四聚体蛋白(68 kDa)。

应用

为了确定 IgG 制剂中孢子中存在的标记多克隆 IgG 的含量。在 300nm 处过量使用已知浓度的 FITC 抗生物素蛋白(来自蛋清和生物素-IgG 抗生物素蛋白)。
来自蛋清的抗生物素蛋白-FITC 已用于:
  • 流式细胞术
  • 双重免疫染色
  • 双重免疫荧光
  • 免疫组织化学

外形

含有 10% (v/v) 0.5 M 碳酸盐缓冲液(pH 9.5) 和 15 mM 叠氮化钠的 0.01 M 磷酸盐缓冲盐水中的溶液。

制备说明

亲和纯化蛋白

免责声明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的人类或动物食用或应用。

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

动植物源性产品

分析证书(COA)

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Anthony Y Huang et al.
British journal of pharmacology, 175(7), 1039-1053 (2018-01-13)
Capsaicin-mediated modulation of taste nerve responses is thought to be produced indirectly by the actions of neuropeptides, for example, CGRP and substance P (SP), on taste cells implying they play a role in taste sensitivity. During the processing of gustatory
Jinjie Liu et al.
Experimental and therapeutic medicine, 19(3), 2103-2112 (2020-02-28)
Numerous cases of spinal cord injury following seizure have been previously reported. However, whether spinal cord injury is a common occurrence after seizures and its underlying mechanisms remain unclear. The present study generated a Sprague-Dawley rat model of temporal lobe
Zanhua Liu et al.
Experimental and therapeutic medicine, 12(2), 695-701 (2016-07-23)
It is well established that brain blood barrier dysfunction following the onset of seizures may lead to serum albumin extravasation into the brain. However, the effect of albumin extravasation on the development of epilepsy is yet to be fully elucidated.
Characterization of a leukocidin identified in Staphylococcus pseudintermedius
Abouelkhair MA, et al.
Testing, 13(9), e0204450-e0204450 (2018)
L Struthers et al.
Analytical biochemistry, 255(1), 20-31 (1998-02-04)
8-Oxodeoxyguanosine is present in DNA from many tissues. The direct demonstration of 8-oxodeoxyguanosine as a potential biomarker of oxidative DNA damage has implications for the study of mutagenesis, carcinogenesis, and free radical toxicity. Avidin is shown here to bind with

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