生物来源
mouse
质量水平
偶联物
agarose conjugate
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
P5D4, monoclonal
形式
PBS suspension
分析物化学类别
proteins (VSV-G)
技术
immunoprecipitation (IP): suitable
protein purification: suitable
同位素/亚型
IgG1
容量
≥15 nmol/mL, resin binding capacity (VSV-G tagged fusion protein)
运输
wet ice
储存温度
2-8°C
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一般描述
Anti-VSV-G Agarose Conjugate is the immunoglobulin fraction of Monoclonal Anti-VSV glycoprotein covalently linked to agarose.
特异性
该抗体可识别包含VSV糖蛋白的五个羧基末端氨基酸的表位。在受感染的细胞中，该抗体将未成熟型VSV-G定位在粗糙内质网（RER）和高尔基复合体潴泡中，将成熟型VSV-G定位在细胞表面和出芽病毒中。该抗体不会染色缺少膜和细胞质结构域的分泌型VSV-G。该抗体可用于微注射抗体、免疫印迹、免疫沉淀、免疫细胞化学和免疫电镜法研究新合成的VSV-G在向质膜和细胞表面转移过程中细胞质结构域的作用。该抗体可用于对以VSV-G羧基末端标记的外源性导入结构进行检测、免疫沉淀和免疫细胞化学染色。该标记不会干扰所研究蛋白质的功能，可被P5D4抗体特异性识别，而不与任何内源性蛋白质发生交叉反应。
免疫原
KLH标记的含有水泡性口炎病毒糖蛋白（VSV-G）的15个羧基末端氨基酸（497-511）的合成肽。
应用
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunoprecipitation (1 paper)
Immunoprecipitation (1 paper)
For immunoprecipitation and affinity purification of VSV-G tagged fusion proteins. The antibody recognizes an epitope containing the five carboxy-terminal amino acids of VSV Glycoprotein.
外形
Supplied as a suspension (1:1,v/v) of beaded agarose in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as preservative
制备说明
Prepared using cyanogen bromide-activated agarose.
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WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Andrew M Lippa et al.
Molecular microbiology, 115(6), 1138-1151 (2020-11-28)
The H-NS-like proteins MvaT and MvaU act coordinately as global repressors in Pseudomonas aeruginosa by binding to AT-rich regions of the chromosome. Although cells can tolerate loss of either protein, identifying their combined regulatory effects has been challenging because the
Yael Katan-Khaykovich et al.
Proceedings of the National Academy of Sciences of the United States of America, 108(4), 1296-1301 (2011-01-12)
Nucleosome deposition occurs on newly synthesized DNA during DNA replication and on transcriptionally active genes via nucleosome-remodeling complexes recruited by activator proteins and elongating RNA polymerase II. It has been long believed that histone deposition involves stable H3-H4 tetramers, such
Sachin Mohan et al.
The Journal of biological chemistry, 285(45), 34566-34578 (2010-08-26)
The small intestinal BB Na(+)/H(+) antiporter NHE3 accounts for the majority of intestinal sodium and water absorption. It is highly regulated with both postprandial inhibition and stimulation sequentially occurring. Phosphatidylinositide 4,5-bisphosphate (PI(4,5)P(2)) and phosphatidylinositide 3,4,5-trisphosphate (PI(3,4,5)P(3)) binding is involved with
Bridget R Kulasekara et al.
eLife, 2, e01402-e01402 (2013-12-19)
Individual cell heterogeneity is commonly observed within populations, although its molecular basis is largely unknown. Previously, using FRET-based microscopy, we observed heterogeneity in cellular c-di-GMP levels. In this study, we show that c-di-GMP heterogeneity in Pseudomonas aeruginosa is promoted by
Larry A Gallagher et al.
Nature microbiology, 7(6), 844-855 (2022-06-02)
DNA-protein interactions are central to fundamental cellular processes, yet widely implemented technologies for measuring these interactions on a genome scale in bacteria are laborious and capture only a snapshot of binding events. We devised a facile method for mapping DNA-protein
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