推荐产品
生物来源
bovine articular cartilage
质量水平
表单
lyophilized powder
包装
glass bottle of 1 mg
技术
cell culture | mammalian: suitable
杂质
salt, essentially free
颜色
white
溶解性
H2O: 2 mg/mL
UniProt登记号
运输
wet ice
储存温度
−20°C
基因信息
cow ... AGC1(280985)
应用
聚集蛋白聚糖是软骨结构和关节功能的关键成分。 研究人员正在研究其合成和降解，以了解其在关节损伤疾病和衰老过程中软骨退化中的作用。
生化/生理作用
这种分子产生一种刚性、可逆、耐压缩的可变形凝胶。 它与透明质酸结合形成非常大的大分子复合物，该复合物具有增加的流体动力学体积和显著增加的溶液相对粘度（30-40%）。
组分
聚集蛋白聚糖是软骨细胞外基质的主要结构蛋白聚糖。 它是一种大蛋白聚糖，分子量大于 2,500 kDa，由连接核心蛋白的大约 100-150 条氨基聚糖链组成。 这些氨基聚糖链大多为软骨素/硫酸皮肤素。聚集蛋白聚糖包含G1、G2 和 G3三个球状结构域，它们与聚集和透明质素（hylauronan ）结合、细胞粘附和软骨细胞凋亡有关。
注意
在 -20°C 储存。按供货时的状态储存，如果储存得当，这种粉末在 3 年内几乎没有分解。
制备说明
该产品从关节软骨中提取，经过化学纯化、水透析，并在冻干前进行0.2 μm过滤。 一旦冻干，这种粉末基本上不含盐。 产品在水中的溶解度为 2 mg/mL。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Association of the aggrecan keratan sulfate-rich region with collagen in bovine articular cartilage.
H Hedlund et al.
The Journal of biological chemistry, 274(9), 5777-5781 (1999-02-20)
Aggrecan, the predominant large proteoglycan of cartilage, is a multidomain macromolecule with each domain contributing specific functional properties. One of the domains contains the majority of the keratan sulfate (KS) chain substituents and a protein segment with a proline-rich hexapeptide
C J Billington et al.
The Biochemical journal, 336 ( Pt 1), 207-212 (1998-11-10)
The breakdown of aggrecan in cartilage is, in part, mediated by an enzyme named aggrecanase that cleaves within the interglobular domain of the molecule between a glutamic residue and an alanine residue. Although the enzyme cleavage site has been identified
L Cao et al.
Matrix biology : journal of the International Society for Matrix Biology, 17(5), 379-392 (1998-11-20)
The proteoglycan aggrecan is a major component of cartilage, and degradation of aggrecan is associated with aging and a number of pathological conditions. To investigate the effects of the accumulation of G1 domain from degraded aggrecan, we overexpressed the G1
M C Bolton et al.
The Biochemical journal, 337 ( Pt 1), 77-82 (1998-12-17)
The rates of incorporation of radiolabelled leucine into aggrecan and link protein have been measured in human articular cartilage of different ages. Aggrecan and link protein were purified in the A1 fraction of CsCl gradients as a result of their
E C Arner et al.
The Journal of biological chemistry, 274(10), 6594-6601 (1999-02-26)
A method was developed for generating soluble, active "aggrecanase" in conditioned media from interleukin-1-stimulated bovine nasal cartilage cultures. Using bovine nasal cartilage conditioned media as a source of the aggrecanase enzyme, an enzymatic assay was established employing purified aggrecan monomers
商品
有五种已发现的糖胺聚糖链（参见图1）透明质酸不是硫酸化的，但其他糖胺聚糖链在链的不同位置含有硫酸根取代基。
Uncover more about glycosaminoglycans and proteoglycans including the structure of glycosaminoglycans (GAGs), the different types of GAGs, and their functions.
Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.
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