A1935
光敏生物素 乙酸盐
For labeling DNA probes for hybridizations
别名:
N-(4-叠氮-2-硝基苯基)-N′-(3-生物素基氨丙基)-N′-甲基-1,3-丙二胺 乙酸盐, 生物素{3-[3-(4-叠氮-2-硝基苯胺)-N-甲基丙氨基]丙酰胺} 乙酸盐
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关于此项目
经验公式（希尔记法）:
C23H35N9O4S · C2H4O2
化学文摘社编号:
分子量:
593.70
NACRES:
NA.52
PubChem Substance ID:
UNSPSC Code:
12352200
MDL number:
InChI
1S/C23H35N9O4S.C2H4O2/c1-31(12-4-10-25-17-9-8-16(29-30-24)14-19(17)32(35)36)13-5-11-26-21(33)7-3-2-6-20-22-18(15-37-20)27-23(34)28-22;1-2(3)4/h8-9,14,18,20,22,25H,2-7,10-13,15H2,1H3,(H,26,33)(H2,27,28,34);1H3,(H,3,4)/t18-,20-,22-;/m0./s1
SMILES string
CC(O)=O.CN(CCCNC(=O)CCCC[C@@H]1SCC2NC(=O)NC12)CCCNc3ccc(cc3[N+]([O-])=O)N=[N+]=[N-]
InChI key
FFBLNTOMOSLSQM-AYEYRVMASA-N
grade
Molecular Biology
assay
≥95%
form
film or powder
solubility
H2O: 10 mg/mL (Stable for at least 5 months if kept frozen and protected from light.)
storage temp.
−20°C
Quality Level
General description
光生物素乙酸盐由生物素组成，通过带电荷连接臂，与光反应性芳基叠氮化物基团结合。
Application
光生物素乙酸盐用于标记DNA探针，进行DNA−DNA杂交 确定基因组相关性。
Biochem/physiol Actions
光生物素乙酸盐能够标记单链DNA和线性或超螺旋双链DNA。
光生物素是可光活化的生物素类似物，主要用于标记DNA、RNA和蛋白。光生物素制备的探针，可检测低至0.5 pg的目标DNA。
Disclaimer
光敏。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Chang Soo Lee et al.
International journal of systematic and evolutionary microbiology, 57(Pt 8), 1775-1779 (2007-08-09)
A novel thiosulfate-oxidizing bacterium, designated strain LCS2(T), was isolated from a biofilm on sulfur particles used in an autotrophic denitrification process. The strain was found to comprise Gram-negative, non-motile, non-spore-forming rods that produced yellow-pigmented colonies on R2A agar. The strain
Preparation and uses of photobiotin
Methods in Enzymology, 184, 588-600 (1990)
Kwang Kyu Kim et al.
International journal of systematic and evolutionary microbiology, 55(Pt 5), 2075-2079 (2005-09-17)
A novel xylan-degrading bacterium, S3-E(T), was isolated from the biofilm of a membrane bioreactor. The cells of this strain were Gram-positive, non-motile, non-spore-forming rods, produced primary branches and formed yellow colonies on nutrient agar. The strain had chemotaxonomic markers that
Long Jin et al.
International journal of systematic and evolutionary microbiology, 57(Pt 8), 1876-1880 (2007-08-09)
Two novel bacteria, strains TR7-09(T) and P2-12-1, were isolated from samples of compost and river sediment, respectively. The strains comprised Gram-negative, motile, non-spore-forming rods, produced creamy white colonies on R2A agar, contained Q-8 as the predominant ubiquinone, contained iso-15 :
Rhodanobacter thiooxydans sp. nov., isolated from a biofilm on sulfur particles used in an autotrophic denitrification process
Lee CS, et al.
International Journal of Systematic and Evolutionary Microbiology, 57(8), 1775-1779 (2007)
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