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Merck
CN

A1603

Sigma-Aldrich

藻酸盐裂解酶

powder, ≥10,000 units/g solid

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别名:
poly-beta-D-mannuronate lyase
CAS号:
MDL编号:
UNSPSC代码:
12352204
NACRES:
NA.54

形式

powder

质量水平

比活

≥10,000 units/g solid

储存温度

2-8°C

一般描述

藻酸盐裂解酶存在于几种海藻、海洋软体动物以及多种海洋和陆地细菌中。

应用

藻酸盐裂解酶可用于分解藻酸盐或海藻酸,也可用于降低粘度。可用于卵泡分离、包封和培养过程中
藻酸盐裂解酶已被用于:
  • 酶的预处理
  • 去封装细胞
  • 消化假单胞菌外多糖,以促进电穿孔

生化/生理作用

藻酸盐裂解酶可积极参与酸性多糖的糖化,以生产生物乙醇。
可将藻酸盐或海藻酸分解成小分子并降低粘度。

单位定义

在37℃、pH 6.3条件下,一单位酶可使每毫升藻酸钠溶液的A235nm每分钟增加1.0

制备说明

将0.15 ml酶溶液(1 un/ml)加到4.5 ml 0.1%藻酸钠(pH 6.3)中。 37℃孵育30分钟。加入4.65 ml 0.1 N NaOH可终止反应。

其他说明

想查看有关 复合碳水化合物分析酶 的更多信息,请访问 www.sigma-aldrich.com/enzymeexplorer

象形图

Health hazard

警示用语:

Danger

危险声明

预防措施声明

危险分类

Resp. Sens. 1

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品

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Lena C E Lundqvist et al.
Carbohydrate research, 352, 44-50 (2012-03-21)
The gene coding for an alginate lyase from the marine bacteria Pseudomonas alginovora X017 was cloned and heterologously expressed in Escherichia coli strains. The protein was produced in inclusion bodies and the active form was obtained by applying a refolding
Adam J Wargacki et al.
Science (New York, N.Y.), 335(6066), 308-313 (2012-01-24)
Prospecting macroalgae (seaweeds) as feedstocks for bioconversion into biofuels and commodity chemical compounds is limited primarily by the availability of tractable microorganisms that can metabolize alginate polysaccharides. Here, we present the discovery of a 36-kilo-base pair DNA fragment from Vibrio
Yuya Morimoto et al.
APL bioengineering, 4(2), 026101-026101 (2020-04-09)
Biohybrid robots composed of biological and synthetic components have been introduced to reconstruct biological functions in mechanical systems and obtain better understanding of biological designs. For example, biohybrid robots powered by skeletal muscle tissue have already succeeded in performing various
Bacteriophages phi MR299-2 and phi NH-4 Can Eliminate Pseudomonas aeruginosa in the Murine Lung and on Cystic Fibrosis Lung Airway Cells
Alemayehu D, et al.
mBio, 3(2) (2012)
Elizabeth M Parrish et al.
Reproduction (Cambridge, England), 142(2), 309-318 (2011-05-26)
Ovarian follicle maturation results from a complex interplay of endocrine, paracrine, and direct cell-cell interactions. This study compared the dynamic expression of key developmental genes during folliculogenesis in vivo and during in vitro culture in a 3D alginate hydrogel system.

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