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一般描述
藻酸盐裂解酶存在于几种海藻、海洋软体动物以及多种海洋和陆地细菌中。
应用
藻酸盐裂解酶可用于分解藻酸盐或海藻酸，也可用于降低粘度。可用于卵泡分离、包封和培养过程中。
藻酸盐裂解酶已被用于：
- 酶的预处理
- 去封装细胞
- 消化假单胞菌外多糖，以促进电穿孔
生化/生理作用
可将藻酸盐或海藻酸分解成小分子并降低粘度。
藻酸盐裂解酶可积极参与酸性多糖的糖化，以生产生物乙醇。
单位定义
在37℃、pH 6.3条件下，一单位酶可使每毫升藻酸钠溶液的A235nm每分钟增加1.0
制备说明
将0.15 ml酶溶液（1 un/ml）加到4.5 ml 0.1％藻酸钠（pH 6.3）中。 37℃孵育30分钟。加入4.65 ml 0.1 N NaOH可终止反应。
其他说明
想查看有关 复合碳水化合物分析酶 的更多信息，请访问 www.sigma-aldrich.com/enzymeexplorer
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
W Hashimoto et al.
Journal of bacteriology, 182(16), 4572-4577 (2000-07-27)
A bacterium, Sphingomonas sp. strain A1, can incorporate alginate into cells through a novel ABC (ATP-binding cassette) transporter system specific to the macromolecule. The transported alginate is depolymerized to di- and trisaccharides by three kinds of cytoplasmic alginate lyases (A1-I
Shinji Sakai et al.
Biomaterials, 33(28), 6721-6727 (2012-07-10)
We report a method for wrapping tissues with a pre-established cage-like layer composed of living cells. We encapsulated multicellular aggregates of human hepatoma HepG2 cells as a model of tissues such as pancreatic islets and hepatocyte spheroids in alginate-based hydrogel
Elizabeth M Parrish et al.
Reproduction (Cambridge, England), 142(2), 309-318 (2011-05-26)
Ovarian follicle maturation results from a complex interplay of endocrine, paracrine, and direct cell-cell interactions. This study compared the dynamic expression of key developmental genes during folliculogenesis in vivo and during in vitro culture in a 3D alginate hydrogel system.
Enzyme-assisted extraction of fucoxanthin and lipids containing polyunsaturated fatty acids from Undaria pinnatifida using dimethyl ether and ethanol
Billakanti JM, et al.
Process Biochemistry (Oxford, United Kingdom), 48(12), 1999-2008 (2013)
Adam J Wargacki et al.
Science (New York, N.Y.), 335(6066), 308-313 (2012-01-24)
Prospecting macroalgae (seaweeds) as feedstocks for bioconversion into biofuels and commodity chemical compounds is limited primarily by the availability of tractable microorganisms that can metabolize alginate polysaccharides. Here, we present the discovery of a 36-kilo-base pair DNA fragment from Vibrio
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