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Merck
CN

A0924

Sigma-Aldrich

丙烯酰胺/N,N'-亚甲基双丙烯酰胺配制试剂

BioReagent, for molecular biology, 49:1 (ratio)

别名:

Acrylamide/Bisacrylamide, 49:1 (2.0% crosslinker), Polyacrylamide solution

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About This Item

MDL编号:
UNSPSC代码:
41105319
PubChem化学物质编号:
NACRES:
NA.25

等级

for molecular biology

质量水平

产品线

BioReagent

形式

powder

投料比

49:1 (ratio)

异质活性

DNase (Exonuclease), NICKase (Endonuclease), RNase, protease, none detected

SMILES字符串

NC(=O)C=C.C=CC(=O)NCNC(=O)C=C

InChI

1S/C7H10N2O2.C3H5NO/c1-3-6(10)8-5-9-7(11)4-2;1-2-3(4)5/h3-4H,1-2,5H2,(H,8,10)(H,9,11);2H,1H2,(H2,4,5)

InChI key

OCQZXMCGTAWGEQ-UHFFFAOYSA-N

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应用

即复溶干粉混合物精确预混合,得到 40% (w/v) 贮备液,用于蛋白质和核酸电泳。该浓度基于丙烯酰胺和双丙烯酰胺的总重量。
Acrylamide/Bis-acrylamide has been used in the electrophoresis of SSCP (single-strand conformation)/heteroduplex.

生化/生理作用

Polyacrylamide gels are prepared using acrylamide and bis-acrylamide, where bis-acrylamide is a cross-linking agent. On the other hand, acrylamide is a water soluble monomer which helps in forming a transparent stable insoluble gel.

特点和优势

无需称量有毒丙烯酰胺和双丙烯酰胺。

重悬

向容器中缓慢加入规定量的蒸馏水复溶,并溶解内容物。
To make up 100 ml of A0924, slowly add 63 ml distilled or deionized water to the container and dissolve contents. Do not divide the solid. This makes 100 ml of a 40% stock solution.
For the 1 Liter size, use 630 mL of water.

象形图

Skull and crossbonesHealth hazard

警示用语:

Danger

危险分类

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral

靶器官

Peripheral nervous system

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges

法规信息

危险化学品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

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在文件库中查找您最近购买产品的文档。

访问文档库

PCR Mutation Detection Protocols.
Guilliatt AM
Methods in Molecular Biology (2002)
Preparation and properties of acrylamide gel for use in electrophoresis.
S RAYMOND et al.
Analytical biochemistry, 1, 391-396 (1960-12-10)
Bhowmik G
Ana Techniqs in Biotechnology (2011)

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