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Merck
CN

A0410

Sigma-Aldrich

氨基烯丙基-dUTP 钠盐

≥85%, lyophilized powder

别名:

5-[3-氨基烯丙基]-2′-脱氧尿苷 5′-三磷酸盐, AA-dUTP

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About This Item

经验公式(希尔记法):
C12H20N3O14P3 · xNa+
分子量:
523.22 (free acid basis)
UNSPSC代码:
41106305
NACRES:
NA.51

质量水平

检测方案

≥85%

形式

lyophilized powder

运输

dry ice

储存温度

−70°C

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应用

氨基烯丙基-dUTP可用于通过聚合酶链式反应(PCR),在第一链cDNA的合成过程中进行间接稳定标记。氨基烯丙基-dUTP可用作反应性位点,用于后续的反应性荧光探针的标记。氨基烯丙基-dUTP也可用于构建反应性DNA支架。
氨基烯丙基-dUTP钠盐已被用于:
  • 添加至脱氧核苷三磷酸混合物中用于互补DNA(cDNA)的合成
  • 生成染色质免疫沉淀(ChIP)探针
  • 在RNA的逆转录中用于合成荧光标记的探针

生化/生理作用

氨基烯丙基-dUTP钠盐/5-(3-氨基烯丙基)-2′-脱氧尿苷5′-三磷酸(aa-dUTP)主要用于合成核酸杂交探针的两步标记法中。

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


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Lorenzo Berti et al.
Nanotechnology, 20(23), 235101-235101 (2009-05-19)
Functional DNA scaffolds can be defined as DNA-based structures comprising chemical moieties facilitating and guiding the immobilization of additional nanocomponents. Due to the limited reactivity of DNA there is currently a need to develop rapid routes to expand its chemical
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Probe production for in situ hybridization by PCR and subsequent covalent labeling with fluorescent dyes.
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Appl. Immunohistochem., 15, 332-337 (2007)
Kevin K Fuller et al.
mBio, 4(2) (2013-03-28)
Light is a pervasive environmental factor that regulates development, stress resistance, and even virulence in numerous fungal species. Though much research has focused on signaling pathways in Aspergillus fumigatus, an understanding of how this pathogen responds to light is lacking.
Effect of the Colorless non-ripening mutation on cell wall biochemistry and gene expression during tomato fruit development and ripening
Eriksson E M, et al.
Plant Physiology, 136(4), 4184-4197 (2004)

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