推荐产品
质量水平
方案
≥85%
表单
lyophilized powder
运输
dry ice
储存温度
−70°C
SMILES字符串
[P](=O)(O[P](=O)(OCC1OC(CC1O)N2C=C(C(=O)NC2=O)\C=C\CN)O)(O[P](=O)(O)O)O.N
InChI key
YWKTUHBZWBRYHX-TYYBGVCCSA-N
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应用
氨基烯丙基-dUTP可用于通过聚合酶链式反应(PCR),在第一链cDNA的合成过程中进行间接稳定标记。氨基烯丙基-dUTP可用作反应性位点,用于后续的反应性荧光探针的标记。氨基烯丙基-dUTP也可用于构建反应性DNA支架。
氨基烯丙基-dUTP钠盐已被用于:
- 添加至脱氧核苷三磷酸混合物中用于互补DNA(cDNA)的合成
- 生成染色质免疫沉淀(ChIP)探针
- 在RNA的逆转录中用于合成荧光标记的探针
生化/生理作用
氨基烯丙基-dUTP钠盐/5-(3-氨基烯丙基)-2′-脱氧尿苷5′-三磷酸(aa-dUTP)主要用于合成核酸杂交探针的两步标记法中。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
历史批次信息供参考:
分析证书(COA)
Effect of the Colorless non-ripening mutation on cell wall biochemistry and gene expression during tomato fruit development and ripening
Plant Physiology, 136(4), 4184-4197 (2004)
Chromatin immunoprecipitation (ChIP) on chip experiments uncover a widespread distribution of NF-Y binding CCAAT sites outside of core promoters
The Journal of Biological Chemistry, 280(14), 13606-13615 (2005)
Probe production for in situ hybridization by PCR and subsequent covalent labeling with fluorescent dyes.
Appl. Immunohistochem., 15, 332-337 (2007)
mBio, 4(2) (2013-03-28)
Light is a pervasive environmental factor that regulates development, stress resistance, and even virulence in numerous fungal species. Though much research has focused on signaling pathways in Aspergillus fumigatus, an understanding of how this pathogen responds to light is lacking.
BioTechniques, 36(1), 114-122 (2004-01-27)
Fluorescent nucleic acid hybridization probes traditionally have been generated by enzymatic incorporation of dye-labeled nucleotides, even though incorporation efficiency is low and variable from dye to dye. Alternatively, 5-(3-aminoallyl)-2'-deoxyuridine 5'-triphosphate (aa-dUTP) is enzymatically incorporated to generate amine-modified DNA, which is
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