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生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
形式
buffered aqueous solution
分子量
antigen ~200 kDa
种属反应性
canine, mouse, rat, human
包装
antibody small pack of 25 μL
增强验证
independent
Learn more about Antibody Enhanced Validation
技术
immunohistochemistry (frozen sections): 1:1,000 using mouse liver sections.
indirect immunofluorescence: 1:500 using MDCK cells and cultured human HepG2 cells.
microarray: suitable
western blot: 1:2,000 using extract of rat brain
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... MLLT4(4301)
mouse ... Mllt4(17356)
rat ... Mllt4(26955)
相关类别
一般描述
Afadin is a multidomain protein that binds F-actin and E-catenin proteins at cell-cell adheren junctions. I-afadin is a ubiquitously expressed splice variant of afadin that contains PDZ and F-actin binding domains at the C-terminal end. I-afadin is homologous to the human AF-6 protein .
特异性
Anti-l-Afadin antibody is specific for I-afadin (approximately 200 kDa). It also binds I-afadin in rat, mouse, dog and human.
免疫原
Synthetic peptide sequence corresponding to amino acid residues 1814-1829 of rat afadin with N-terminal cysteine conjugated to maleimide-activated KLH.
应用
Anti-l-Afadin antibody produced in rabbit has been used in:
- western blotting
- immunofluorescence experiments
- immunocytochemistry
- immunohistochemistry
生化/生理作用
AF-6/Afadin regulates the formation of tight and adherens junctions in the cell and its absence in mice leads to embryonic defects and lethality . Afadin is implicated in primary breast cancer and pancreatic cancer. It downregulates the process of migration and metastasis in cancer cells.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
含少量动物源组分生物产品
常规特殊物品
Afadin: a key molecule essential for structural organization of cell-cell junctions of polarized epithelia during embryogenesis
Ikeda W, et al.
The Journal of cell biology, 146(5), 1117-1132 (1999)
Lauren M Popov et al.
Proceedings of the National Academy of Sciences of the United States of America, 112(46), 14337-14342 (2015-10-23)
Staphylococcus aureus is both a transient skin colonizer and a formidable human pathogen, ranking among the leading causes of skin and soft tissue infections as well as severe pneumonia. The secreted bacterial α-toxin is essential for S. aureus virulence in
Mechanosensitive EPLIN-dependent remodeling of adherens junctions regulates epithelial reshaping
Taguchi, K. et. Al
The Journal of Cell Biology, 194, 634-656 (2011)
Afadin cooperates with Claudin-2 to promote breast cancer metastasis
Tabaries S, et al.
Genes & Development, 33(3-4), 180-193 (2019)
A genome-wide screen identifies conserved protein hubs required for cadherin-mediated cell-cell adhesion
Toret CP, et al.
The Journal of cell biology, 204(2), 265-279 (2014)
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