生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
分子量
antigen ~200 kDa (and 190 kDa)
种属反应性
rat, human, canine, mouse
增强验证
independent
Learn more about Antibody Enhanced Validation
技术
immunocytochemistry: suitable
immunohistochemistry (frozen sections): 1:1,000 using mouse liver sections.
indirect immunofluorescence: 1:250 using cultured dog (MDCK) cells and of cultured human HepG2 cells
indirect immunofluorescence: 1:500 using frozen sections of mouse liver
microarray: suitable
western blot: 1:1000 using extract of rat brain.
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... MLLT4(4301)
mouse ... Mllt4(17356)
rat ... Mllt4(26955)
相关类别
一般描述
Afadin is a cell-cell adherens junction, F-actin binding multidomain protein. This adherens junction protein has two splicing variants: the ubiquitously expressed l-afadin, and the smaller s-afadin, which is abundantly expressed in neural tissues. Afadin associates with E-catenin proteins.
S-Afadin is a protein which lacks both the third proline-rich and the F-actin binding regions and is homologous to the human AF-6 (ALL-1 fusion partner from chromosome 6) gene product. Anti-l/s-Afadin is developed in rabbit using a synthetic peptide of rat afadin.
特异性
Anti-l/s-Afadin specifically binds to l-afadin and s-afadin by immunoblotting (approximately 200 and 190 kDa respectively). Additional bands may appear in certain extract preparations in western blot assays. The immunizing peptide specifically inhibits the staining of the I/s-afadin bands. The antibody is specific for human, dog, rat, and mouse l/s-afadin.
免疫原
synthetic peptide corresponding to rat l/s afadin sequence, amino acids 1163-1179, with N-terminal cysteine added, conjugated to BSA.
应用
Anti-l/s-Afadin antibody produced in rabbit has been used in :
- immunocytochemistry
- immunohistochemistry
- immunoprecipitation
生化/生理作用
s-Afadin binds to several cytoplasmic proteins such as the small guanosine triphosphatase (GTPase) Ras, the tight junction protein ZO-1 2 and the vinculin binding protein ponsin/ sorbin and SH3 domain containing 1 (SH3P12). In addition, l-afadin also binds through its PDZ several integral membrane components such as the Ca2+ -independent homophilic immunoglobulin-like nectin, the junctional adhesion molecule (JAM)4 and a subset of the EphB receptor protein-tyrosine kinases.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
含少量动物源组分生物产品
Morten Lundh et al.
Scientific reports, 11(1), 9794-9794 (2021-05-09)
The profound energy-expending nature of brown adipose tissue (BAT) thermogenesis makes it an attractive target tissue to combat obesity-associated metabolic disorders. While cold exposure is the strongest inducer of BAT activity, the temporal mechanisms tuning BAT adaptation during this activation
Afadin: A novel actin filament-binding protein with one PDZ domain localized at cadherin-based cell-to-cell adherens junction
Mandai K, et al.
The Journal of cell biology, 139(2), 517-528 (1997)
Afadin is required for maintenance of dendritic structure and excitatory tone
Srivastava DP, et al.
The Journal of Biological Chemistry, 287(43), 35964-35974 (2012)
The junction-associated protein AF-6 interacts and clusters with specific Eph receptor tyrosine kinases at specialized sites of cell-cell contact in the brain
Buchert M, et al.
The Journal of cell biology, 144(2), 361-371 (1999)
Lei Gao et al.
Development (Cambridge, England), 144(19), 3511-3520 (2017-09-02)
In many types of tubules, continuity of the lumen is paramount to tubular function, yet how tubules generate lumen continuity in vivo is not known. We recently found that the F-actin-binding protein afadin is required for lumen continuity in developing
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