biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 80-100 kDa
species reactivity
human
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
concentration
~1 mg/mL
technique(s)
immunoprecipitation (IP): 10-20 μg using TGN46 from human HepG2 cell lysates., indirect immunofluorescence: 5-10 μg/mL using human A549 cells, western blot (chemiluminescent): 0.25-0.5 μg/mL using extracts of human HEK-293T cells expressing recombinant human TGN46
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... TGOLN2(10618)
General description
TGN46(反式高尔基体网络蛋白,46 kDa)是大鼠TGN38的人类同源物,是反式高尔基体网络(TGN)的固有整合膜蛋白,其可在TGN和质膜之间进行组成性循环,并通过内体返回。TGN是蛋白质分泌途径、脂质和膜运输的的主要分选细胞器。TGN46是一种高度糖基化的蛋白质,可能参与调节往返TGN的膜运输。TGN46包含信号肽、内腔结构域、跨膜结构域和胞质结构域。跨膜区域和胞质尾巴分别包含保留和回收信号,可用于TGN的定位。TGN46在细胞中广泛表达。
抗TGN46可识别人反式高尔基体网络蛋白2(TGN46)。通过免疫印迹对TGN46条带的检测可被免疫肽特异性抑制。
Immunogen
对应于人TGN46的氨基酸残基426-437的合成肽,N-末端添加有半胱氨酸,并与KLH偶联。与大鼠中的相应序列相同,与人的相应序列相差2个氨基酸。
Application
兔多克隆抗TGN46抗体可用于标记反式高尔基体网络蛋白2,并可通过免疫细胞化学和免疫组化(IHC)技术(例如免疫印迹、免疫沉淀和免疫荧光)进行检测和定量。它可作为探针,对反式高尔基网络蛋白2[作为反式高尔基网络(TGN)的膜转运调节成分]的存在和作用进行确定。抗TGN46可用作TGN标记。
Physical form
0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、体外或体内治疗用途,或人类或动物任何类型的消费或使用。
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
低风险生物材料
常规特殊物品
此项目有
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| T7576-200UL | 04061838037992 |