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应用
溶壁微球菌ATCC号4698，已被用于一项评估中空纤维超滤分离溶菌酶的研究中。 它也被用于研究可生物降解微粒中蛋白质药物的封装研究。
从溶壁微球菌培养物中收获的溶菌酶裂解物被附着在琼脂糖凝胶上并用于亲和层析以分离各种溶菌酶。
生化/生理作用
藤黄微球菌(Micrococcus luetus)是一种革兰氏阳性菌，可通过释放水不溶性黄色素加以鉴定。这种细菌生长需要琥珀酸，并易被产酶溶杆菌(Lysobacter enzymogenes)通过β-水解金属内肽酶(β-lytic metalloendopeptidase)裂解。它的膜中含有一种酶，可以异戊二烯焦磷酸为供体进行异戊二烯化反应。藤黄微球菌可用于cis-异戊烯基转移酶的基因克隆。
质量
含多核苷酸磷酸化酶。
单位定义
One unit will lyse 0.6 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 6.2 at 25 °C.
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
高风险级别生物产品--病毒，疫苗等
从最新的版本中选择一种：
分析证书(COA)
Lot/Batch Number
Generation of a reference transcriptome for evaluating rainbow trout responses to various stressors.
Cecilia C Sánchez et al.
BMC genomics, 12, 626-626 (2011-12-23)
Fish under intensive culture conditions are exposed to a variety of acute and chronic stressors, including high rearing densities, sub-optimal water quality, and severe thermal fluctuations. Such stressors are inherent in aquaculture production and can induce physiological responses with adverse
Tania Jauslin et al.
mBio, 12(1) (2021-02-18)
Ingestion and killing of bacteria by phagocytic cells protect the human body against infections. While many mechanisms have been proposed to account for bacterial killing in phagosomes, their relative importance, redundancy, and specificity remain unclear. In this study, we used
Tracking molecular recognition at the atomic level with a new protein scaffold based on the OB-fold.
John D Steemson et al.
PloS one, 9(1), e86050-e86050 (2014-01-28)
The OB-fold is a small, versatile single-domain protein binding module that occurs in all forms of life, where it binds protein, carbohydrate, nucleic acid and small-molecule ligands. We have exploited this natural plasticity to engineer a new class of non-immunoglobulin
Marion Le Coadic et al.
PloS one, 8(1), e53259-e53259 (2013-01-10)
Dictyostelium discoideum has largely been used to study phagocytosis and intracellular killing of bacteria. Previous studies have shown that Phg1A, Kil1 and Kil2 proteins are necessary for efficient intracellular killing of Klebsiella bacteria. Here we show that in phg1a KO
J C Cox et al.
Bioorganic & medicinal chemistry, 9(10), 2525-2531 (2001-09-15)
The in vitro selection of nucleic acid binding species (aptamers) is frequently repetitive, time-consuming, and poorly adapted to high-throughput applications. We have adapted automated workstations to select anti-protein aptamers; as an example, we demonstrated the selection of anti-lysozyme aptamers that
实验方案
To measure achromopeptidase activity, this procedure uses bacterial cells and a turbidimetric rate assay. Turbidity is measured at 600 nm using a spectrophotometer.
This enzymatic rate determination may be used for Lysozyme products. It is not to be used to assay recombinant or insoluble Lysozyme on agarose.
该酶速率测定可用于溶菌酶产物。它不适用于在测定在琼脂糖上的重组型或不溶性溶菌酶。
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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