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Designed and tested for fluorescent super-resolution microscopy
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abberior is a registered trademark of Abberior GmbH
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WGK 3
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新产品
Marcus Dyba et al.
Nature biotechnology, 21(11), 1303-1304 (2003-10-21)
We report immunofluorescence imaging with a spatial resolution well beyond the diffraction limit. An axial resolution of approximately 50 nm, corresponding to 1/16 of the irradiation wavelength of 793 nm, is achieved by stimulated emission depletion through opposing lenses. We
T A Klar et al.
Optics letters, 24(14), 954-956 (2007-12-13)
We overcame the resolution limit of scanning far-field fluorescence microscopy by disabling the fluorescence from the outer part of the focal spot. Whereas a near-UV pulse generates a diffraction-limited distribution of excited molecules, a spatially offset pulse quenches the excited
Tim Grotjohann et al.
Nature, 478(7368), 204-208 (2011-09-13)
Lens-based optical microscopy failed to discern fluorescent features closer than 200 nm for decades, but the recent breaking of the diffraction resolution barrier by sequentially switching the fluorescence capability of adjacent features on and off is making nanoscale imaging routine. Reported
S W Hell et al.
Optics letters, 19(11), 780-782 (1994-06-01)
We propose a new type of scanning fluorescence microscope capable of resolving 35 nm in the far field. We overcome the diffraction resolution limit by employing stimulated emission to inhibit the fluorescence process in the outer regions of the excitation
Stefan W Hell
Nature biotechnology, 21(11), 1347-1355 (2003-11-05)
For more than a century, the resolution of focusing light microscopy has been limited by diffraction to 180 nm in the focal plane and to 500 nm along the optic axis. Recently, microscopes have been reported that provide three- to
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