质量水平
检测方案
≥85% (HPLC)
形式
powder
溶解性
DMSO: soluble
methanol: soluble
荧光
λex 543 nm; λem 575 nm in 0.1 M phosphate pH 7.0
适用性
suitable for fluorescence
储存温度
−20°C
InChI
1S/C28H25N3O5/c1-29(2)16-5-9-20-23(14-16)36-24-15-17(30(3)4)6-10-21(24)27(20)19-8-7-18(13-22(19)28(34)35)31-25(32)11-12-26(31)33/h5-9,11-15H,10H2,1-4H3,(H,34,35)
InChI key
KGFLZYXDJDOIEE-UHFFFAOYSA-N
相关类别
应用
使用四甲基罗丹明-5-马来酰亚胺：
- 浓缩洗脱液并进行标记创建IRE1LDS112C
- 利用外源荧光探针在实验用Cys48上衍生绿色荧光蛋白(GFP)
- 培养单溶剂显露的半胱氨酸突变以进行蛋白质标记
包装
无底玻璃瓶。内容物在插入式融锥内。
其他说明
用作细胞荧光分析中蛋白质硫醇的标记试剂
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
A J-protein co-chaperone recruits BiP to monomerize IRE1 and repress the unfolded protein response
Amin-WN, et al.
Cell, 171(7), 1625-1637 (2017)
Effect of the point mutation H148G on GFPmut2 unfolding kinetics by fluorescence spectroscopy
Bosisio C, et al.
Biophysical Chemistry, 157(1-3), 24-32 (2011)
Cytofluorescence techniques for the visualization of distinct pools of protein thiols at the single cell level.
Alfonso Pompella et al.
Methods in molecular biology (Clifton, N.J.), 196, 305-312 (2002-08-03)
Specific stereochemistry of OP-1074 disrupts estrogen receptor alpha helix 12 and confers pure antiestrogenic activity
Fanning SW, et al.,
Nature Communications, 9(1), 2368-2368 (2018)
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