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一般描述
亚基分子量：约 90,770
由六个亚基组成，总分子量：约 544620
由六个亚基组成，总分子量：约 544620
脲酶（Ure）酶存在于胞质溶胶中。它在酸性 pH 下具有活性，在 28℃ 下表达。脲酶由镍组成，存在于古细菌、细菌、单细胞真核生物和植物中。
应用
来自洋刀豆的脲酶可用于各种样品的尿素测定，例如豆类。它可用于检测病原体以及重金属离子。
来自洋刀豆（Jack bean）的脲酶已用于纯化和结晶研究。
生化/生理作用
脲酶催化尿素水解成二氧化碳和氨。脲酶参与氮代谢和尿素降解。来自洋刀豆的脲酶每个亚基结合 2 个镍离子。
单位定义
1U 对应于在 pH 8.0 和 25℃ 下每分钟水解 1 μmol 尿素（货号 51459）所需的酶量。
其他说明
在（选择性）水解/缩合中的应用；结构。
警示用语：
Danger
危险分类
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
储存分类代码
11 - Combustible Solids
WGK
WGK 1
个人防护装备
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
法规信息
动植物源性产品
K Takishima et al.
European journal of biochemistry, 175(1), 151-165 (1988-07-15)
The amino acid sequence of jack bean urease has been determined. The protein consists of a single kind of polypeptide chain containing 840 amino acid residues. The subunit relative molecular mass calculated from the sequence is 90,770, indicating that urease
M J Sculley et al.
Biophysical chemistry, 19(1), 39-47 (1984-01-01)
The use of bifunctional reagents to form cross-links between subunits in protein oligomers and subsequent disruption of noncovalent interactions with SDS allows comment upon the number of subunits and the symmetry in the original assembly. In existing treatments the number
Isothermal titration calorimetry to characterize enzymatic reactions
Methods in Enzymology, 567, 215-236 (2016)
I.A. Natchev
Tetrahedron, 44, 1511-1511 (1988)
Brendan Wallace et al.
Journal of endourology, 37(3), 358-366 (2022-12-24)
Introduction: The pathogenesis of infectious kidney stones is poorly understood. Bacteria have been implicated in promoting infectious stones via urease production; however, there is mounting evidence indicating the relationship is more complex. The aim of our study was to characterize
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