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Merck
CN

93711

Sigma-Aldrich

Atto 655

BioReagent, suitable for fluorescence, ≥85% (HPLC)

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About This Item

MDL编号:
UNSPSC代码:
12352116
PubChem化学物质编号:
NACRES:
NA.32

产品线

BioReagent

质量水平

方案

≥85% (HPLC)

表单

powder

制造商/商品名称

ATTO-TEC GmbH

透射比

254 nm
655 nm

荧光

λex 655 nm; λem 680 nm in 0.1 M phosphate pH 7.0

λ

in ethanol (with 0.1% trifluoroacetic acid)

紫外吸收

λ: 652-658 nm Amax

适用性

suitable for fluorescence

储存温度

−20°C

SMILES字符串

[S](=O)(=O)([O-])CC1CC(N(c2c1cc3c(c2)OC4=CC5=[N+](CCCC5=CC4=N3)CC)CCCC(=O)O)(C)C

InChI

1S/C27H33N3O6S/c1-4-29-9-5-7-17-11-20-24(13-22(17)29)36-25-14-23-19(12-21(25)28-20)18(16-37(33,34)35)15-27(2,3)30(23)10-6-8-26(31)32/h11-14,18H,4-10,15-16H2,1-3H3,(H-,31,32,33,34,35)

InChI key

FOYVTVSSAMSORJ-UHFFFAOYSA-N

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应用

Atto 标签适用于灵敏度最高的应用。独特的优势组合使其成为各种标签应用的极佳工具。它们的一些特性使它们特别适用于单分子检测。Atto 标签基于刚性结构,并且不显示任何顺式-反式-异构化,这降低了信号的亮度,并导致环境依赖性,例如共轭光谱移位。
Atto 655 在水中摩尔消光为 110,000,在含 50% 乙醇的水溶液中 QY 为 30%。衰变时间是 1.9 ns。

其他说明

新型红色吸收荧光染料,具有最佳的信噪比和长荧光寿命。可用作结合相互作用的生物物理探针。

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储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


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Volker Buschmann et al.
Bioconjugate chemistry, 14(1), 195-204 (2003-01-16)
The spectroscopic characteristics (absorption, emission, and fluorescence lifetime) of 13 commercially available red-absorbing fluorescent dyes were studied under a variety of conditions. The dyes included in this study are Alexa647, ATTO655, ATTO680, Bodipy630/650, Cy5, Cy5.5, DiD, DY-630, DY-635, DY-640, DY-650
Bengang Xing et al.
Chemistry (Weinheim an der Bergstrasse, Germany), 17(50), 14170-14177 (2011-11-16)
The molecular interactions of the glycopeptide antibiotic vancomycin (Van) with bacterial cell wall analogues N,N'-diacetyl-L-Lys-D-Ala-D-Ala (Ac(2) KdAdA) and N,N'-diacetyl-L-Lys-D-Ala-D-Lac (Ac(2) KdAdL) were investigated in neat water, phosphate buffer and HEPES buffer by using fluorescence correlation spectroscopy (FCS) and molecular dynamics
Søren Preus et al.
Chembiochem : a European journal of chemical biology, 13(14), 1990-2001 (2012-09-01)
Förster resonance energy transfer (FRET) is a powerful tool for monitoring molecular distances and interactions at the nanoscale level. The strong dependence of transfer efficiency on probe separation makes FRET perfectly suited for "on/off" experiments. To use FRET to obtain
Achim Friedrich et al.
FEBS letters, 581(8), 1644-1648 (2007-04-03)
This article presents a new, highly sensitive method for the identification of single nucleotide polymorphisms (SNPs) in homogeneous solutions using fluorescently labeled hairpin-structured oligonucleotides (smart probes) and fluorescence single-molecule spectroscopy. While the hairpin probe is closed, fluorescence intensity is quenched
Eilon Sherman et al.
Chemphyschem : a European journal of chemical physics and physical chemistry, 12(3), 696-703 (2011-01-29)
Intramolecular dynamics in the denatured state of a protein are of importance for protein folding. Native-like contact formation within the ensemble of denatured conformations of a protein may guide its transformation towards the native conformation. The efficiency of folding is

商品

荧光寿命测量与基于强度的测量相比是有优势的。其应用包括荧光寿命分析,感测和FLI。

Fluorescence lifetime measurement is advantageous over intensity-based measurements. Applications include fluorescence lifetime assays, sensing and FLI.

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