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Merck
CN

93711

Atto 655

BioReagent, suitable for fluorescence, ≥85% (HPLC)

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关于此项目

UNSPSC Code:
12352116
PubChem Substance ID:
57653573
NACRES:
NA.32
MDL number:
MFCD03456136

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产品名称

Atto 655, BioReagent, suitable for fluorescence, ≥85% (HPLC)

SMILES string

[S](=O)(=O)([O-])CC1CC(N(c2c1cc3c(c2)OC4=CC5=[N+](CCCC5=CC4=N3)CC)CCCC(=O)O)(C)C

InChI

1S/C27H33N3O6S/c1-4-29-9-5-7-17-11-20-24(13-22(17)29)36-25-14-23-19(12-21(25)28-20)18(16-37(33,34)35)15-27(2,3)30(23)10-6-8-26(31)32/h11-14,18H,4-10,15-16H2,1-3H3,(H-,31,32,33,34,35)

InChI key

FOYVTVSSAMSORJ-UHFFFAOYSA-N

product line

BioReagent

assay

≥85% (HPLC)

form

powder

manufacturer/tradename

ATTO-TEC GmbH

transmittance

254 nm
655 nm

fluorescence

λex 655 nm; λem 680 nm in 0.1 M phosphate pH 7.0

λ

in ethanol (with 0.1% trifluoroacetic acid)

UV absorption

λ: 652-658 nm Amax

suitability

suitable for fluorescence

storage temp.

−20°C

Quality Level

100

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Application

Atto 标签适用于灵敏度最高的应用。独特的优势组合使其成为各种标签应用的极佳工具。它们的一些特性使它们特别适用于单分子检测。Atto 标签基于刚性结构，并且不显示任何顺式-反式-异构化，这降低了信号的亮度，并导致环境依赖性，例如共轭光谱移位。
Atto 655 在水中摩尔消光为 110,000，在含 50% 乙醇的水溶液中 QY 为 30％。衰变时间是 1.9 ns。

Other Notes

新型红色吸收荧光染料，具有最佳的信噪比和长荧光寿命。可用作结合相互作用的生物物理探针。

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
BCCP0768
BCCN8603
BCCN5830
BCCN4662
BCCN3519

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Volker Buschmann et al.
Bioconjugate chemistry, 14(1), 195-204 (2003-01-16)
The spectroscopic characteristics (absorption, emission, and fluorescence lifetime) of 13 commercially available red-absorbing fluorescent dyes were studied under a variety of conditions. The dyes included in this study are Alexa647, ATTO655, ATTO680, Bodipy630/650, Cy5, Cy5.5, DiD, DY-630, DY-635, DY-640, DY-650
Eilon Sherman et al.
Chemphyschem : a European journal of chemical physics and physical chemistry, 12(3), 696-703 (2011-01-29)
Intramolecular dynamics in the denatured state of a protein are of importance for protein folding. Native-like contact formation within the ensemble of denatured conformations of a protein may guide its transformation towards the native conformation. The efficiency of folding is
Julie M G Rogers et al.
Langmuir : the ACS journal of surfaces and colloids, 27(7), 3815-3821 (2011-03-16)
The structure and function of the influenza A M2 proton channel have been the subject of intensive investigations in recent years because of their critical role in the life cycle of the influenza virus. Using a truncated version of the
Achim Friedrich et al.
FEBS letters, 581(8), 1644-1648 (2007-04-03)
This article presents a new, highly sensitive method for the identification of single nucleotide polymorphisms (SNPs) in homogeneous solutions using fluorescently labeled hairpin-structured oligonucleotides (smart probes) and fluorescence single-molecule spectroscopy. While the hairpin probe is closed, fluorescence intensity is quenched
Ana J García-Sáez et al.
The Journal of biological chemistry, 286(43), 37768-37777 (2011-09-03)
Pore-forming toxins have evolved to induce membrane injury by formation of pores in the target cell that alter ion homeostasis and lead to cell death. Many pore-forming toxins use cholesterol, sphingolipids, or other raft components as receptors. However, the role
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商品

Fluorescence Lifetime Measurement

Fluorescence lifetime measurement is advantageous over intensity-based measurements. Applications include fluorescence lifetime assays, sensing and FLI.

荧光寿命测量

荧光寿命测量与基于强度的测量相比是有优势的。其应用包括荧光寿命分析，感测和FLI。

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