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Merck
CN

92846

Sigma-Aldrich

5-羧基荧光素 N -琥珀酰亚胺酯

BioReagent, suitable for fluorescence, ≥90% (HPLC)

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About This Item

经验公式(希尔记法):
C25H15NO9
CAS号:
分子量:
473.39
Beilstein:
7865828
MDL编号:
UNSPSC代码:
12352108
PubChem化学物质编号:
NACRES:
NA.32

产品线

BioReagent

质量水平

方案

≥90% (HPLC)

表单

powder

荧光

λex 492 nm; λem 520 nm in 0.1 M Tris pH 8.0

适用性

suitable for fluorescence

储存温度

−20°C

SMILES字符串

OC(=O)c1cc(ccc1C2=C3C=CC(=O)C=C3Oc4cc(O)ccc24)C(=O)ON5C(=O)CCC5=O

InChI

1S/C25H15NO9/c27-13-2-5-16-19(10-13)34-20-11-14(28)3-6-17(20)23(16)15-4-1-12(9-18(15)24(31)32)25(33)35-26-21(29)7-8-22(26)30/h1-6,9-11,27H,7-8H2,(H,31,32)

InChI key

TWOKNYRPKCLMAR-UHFFFAOYSA-N

包装

无底玻璃瓶。内含物装在插入的融合锥内。

其他说明

荧光素衍生物在与生物聚合物偶联时产生稳定的衍生物。核苷酸的荧光探针 ;毛细管电泳荧光衍生试剂

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储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


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Lau, S.K., et al.
Journal of Chromatography A, 809, 203-203 (1998)
G L Igloi
Analytical biochemistry, 233(1), 124-129 (1996-01-01)
A modified ribodinucleotide (named pSEEp) has been synthesized using commercially available components on a DNA synthesizer. The presence of a 3'-terminal primary amino group permits its coupling to a range of nonradioactive labels, exemplified here by fluorescein. The product of
Bamaprasad Dutta et al.
Scientific reports, 7(1), 5765-5765 (2017-07-20)
Atherosclerosis arises from leukocyte infiltration and thickening of the artery walls and constitutes a major component of vascular disease pathology, but the molecular events underpinning this process are not fully understood. Proteins containing an Asn-Gly-Arg (NGR) motif readily undergo deamidation
L G Lee et al.
Nucleic acids research, 20(10), 2471-2483 (1992-05-25)
The incorporation of fluorescently labeled dideoxynucleotides by T7 DNA polymerase is optimized by the use of Mn2+, fluorescein analogs and four 2'-deoxyribonucleoside 5'-O-(1-thiotriphosphates) (dNTP alpha S's). The one-tube extension protocol was tested on single-stranded templates, as well as PCR fragments
Brandon L Blakely et al.
Nature methods, 11(12), 1229-1232 (2014-10-13)
We developed molecular tension probes (TPs) that report traction forces of adherent cells with high spatial resolution, can in principle be linked to virtually any surface, and obviate monitoring deformations of elastic substrates. TPs consist of DNA hairpins conjugated to

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