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经验公式(希尔记法):
C25H15NO9
化学文摘社编号:
分子量:
473.39
UNSPSC Code:
12352108
NACRES:
NA.32
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
7865828
产品名称
5-羧基荧光素 N -琥珀酰亚胺酯, BioReagent, suitable for fluorescence, ≥90% (HPLC)
InChI
1S/C25H15NO9/c27-13-2-5-16-19(10-13)34-20-11-14(28)3-6-17(20)23(16)15-4-1-12(9-18(15)24(31)32)25(33)35-26-21(29)7-8-22(26)30/h1-6,9-11,27H,7-8H2,(H,31,32)
SMILES string
OC(=O)c1cc(ccc1C2=C3C=CC(=O)C=C3Oc4cc(O)ccc24)C(=O)ON5C(=O)CCC5=O
InChI key
TWOKNYRPKCLMAR-UHFFFAOYSA-N
product line
BioReagent
assay
≥90% (HPLC)
form
powder
fluorescence
λex 492 nm; λem 520 nm in 0.1 M Tris pH 8.0
suitability
suitable for fluorescence
storage temp.
−20°C
Quality Level
Other Notes
荧光素衍生物在与生物聚合物偶联时产生稳定的衍生物。核苷酸的荧光探针 ;毛细管电泳荧光衍生试剂
Packaging
无底玻璃瓶。内含物装在插入的融合锥内。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Lau, S.K., et al.
Journal of Chromatography A, 809, 203-203 (1998)
G L Igloi
Analytical biochemistry, 233(1), 124-129 (1996-01-01)
A modified ribodinucleotide (named pSEEp) has been synthesized using commercially available components on a DNA synthesizer. The presence of a 3'-terminal primary amino group permits its coupling to a range of nonradioactive labels, exemplified here by fluorescein. The product of
Bamaprasad Dutta et al.
Scientific reports, 7(1), 5765-5765 (2017-07-20)
Atherosclerosis arises from leukocyte infiltration and thickening of the artery walls and constitutes a major component of vascular disease pathology, but the molecular events underpinning this process are not fully understood. Proteins containing an Asn-Gly-Arg (NGR) motif readily undergo deamidation
L G Lee et al.
Nucleic acids research, 20(10), 2471-2483 (1992-05-25)
The incorporation of fluorescently labeled dideoxynucleotides by T7 DNA polymerase is optimized by the use of Mn2+, fluorescein analogs and four 2'-deoxyribonucleoside 5'-O-(1-thiotriphosphates) (dNTP alpha S's). The one-tube extension protocol was tested on single-stranded templates, as well as PCR fragments
Brandon L Blakely et al.
Nature methods, 11(12), 1229-1232 (2014-10-13)
We developed molecular tension probes (TPs) that report traction forces of adherent cells with high spatial resolution, can in principle be linked to virtually any surface, and obviate monitoring deformations of elastic substrates. TPs consist of DNA hairpins conjugated to
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