产品名称
Atto 532 NHS ester, BioReagent, suitable for fluorescence, ≥90% (HPLC)
product line
BioReagent
assay
≥90% (HPLC)
≥90% (degree of coupling)
form
powder
manufacturer/tradename
ATTO-TEC GmbH
λ
in methanol: water (1:1) (with 0.1% perchloric acid)
UV absorption
λ: 532-538 nm Amax
suitability
suitable for fluorescence
storage temp.
−20°C
Quality Level
Application
Atto 532 NHS ester is highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, and STED. In addition, the dye is used in flow cytometry (FACS) and fluorescence in-situ hybridization (FISH) methods.
Features and Benefits
Characteristic features of the Atto 532 NHS ester are:
- Strong Absorption.
- High Fluorescence quantum yield.
- High Photostability.
- Excellent water solubility.
General description
Atto 532 NHS ester is a fluorescent dye related to the well-known laser dye, Rhodamine 6G. The fluorescence activity is excited efficiently at the 515-545nm range. A suitable excitation source for Atto 532 is the 532 nm output of the frequency-doubled Nd: YAG laser.
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Rumelo Amor et al.
Scientific reports, 4, 7359-7359 (2014-12-09)
Standing-wave excitation of fluorescence is highly desirable in optical microscopy because it improves the axial resolution. We demonstrate here that multiplanar excitation of fluorescence by a standing wave can be produced in a single-spot laser scanning microscope by placing a
Wonchul Shin et al.
Cell, 173(4), 934-945 (2018-04-03)
Fusion is thought to open a pore to release vesicular cargoes vital for many biological processes, including exocytosis, intracellular trafficking, fertilization, and viral entry. However, fusion pores have not been observed and thus proved in live cells. Its regulatory mechanisms and
Roland Bienert et al.
Chemphyschem : a European journal of chemical physics and physical chemistry, 12(3), 510-517 (2011-02-03)
H(+)-ATP synthases are molecular machines which couple transmembrane proton transport with ATP synthesis from ADP and inorganic phosphate by a rotational mechanism. Single-pair fluorescence resonance energy transfer (spFRET) in single molecules is a powerful tool to analyse conformational changes. It
SERRS-based detection of dye-labeled DNA using positively-charged Ag nanoparticles.
Gill, R. and Lucassen, G. W.
Analytical Methods : Advancing Methods and Applications, 2, 445-447 (2010)
Stefan Bretschneider et al.
Physical review letters, 98(21), 218103-218103 (2007-08-07)
We report the breaking of the diffraction resolution barrier in far-field fluorescence microscopy by transiently shelving the fluorophore in a metastable dark state. Using a relatively modest light intensity of several kW/cm(2) in a focal distribution featuring a local zero
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