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Merck
CN

87748

Sigma-Aldrich

3,3′,5,5′-四甲基联苯胺

TMB substrate, chromogenic, ≥98.0% (NT), powder

别名:

BM蓝, Sure Blue TMB, TMB, TMB印迹Plus, TMB底物

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About This Item

线性分子式:
[-C6H2(CH3)2-4-NH2]2
CAS号:
分子量:
240.34
Beilstein:
2808541
EC 号:
MDL编号:
UNSPSC代码:
12352204
PubChem化学物质编号:
NACRES:
NA.83

product name

3,3′,5,5′-四甲基联苯胺, ≥98.0% (NT)

质量水平

检测方案

≥98.0% (NT)

形式

powder

技术

UV/Vis spectroscopy: suitable

mp

166-171 °C
168-171 °C (lit.)

储存温度

2-8°C

SMILES字符串

Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2

InChI

1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3

InChI key

UAIUNKRWKOVEES-UHFFFAOYSA-N

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相关类别

应用

3,3′,5,5′-四甲基联苯胺已用作以下应用的过氧物酶底物:
  • 酶免疫测定(EIA)
  • 间接竞争法酶联免疫吸附测定(ELISA)
  • 三明治法酶联免疫吸附测定

3,3′,5,5′-四甲基联苯胺(TMB)是一种二氨基联苯胺的非致癌性取代物(Ames测试阴性)适合在ELISA实验中作为过氧化物酶底物。底物产生可溶性终产物,淡蓝色,分光光度检测在370或620-650nm可以读峰值。TMB反应能被2M H2SO4终止(产生黄色),在450nm处可读。一种高敏感,特异性试剂用于检测血液,血红蛋白实验,过氧化物酶实验。

象形图

Health hazardExclamation mark

警示用语:

Warning

危险声明

危险分类

Acute Tox. 4 Oral - Aquatic Chronic 4 - Carc. 2

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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