等级
for molecular biology
质量水平
形式
solid
分子量
Mr 5000-8000
杂质
≤10% water
溶解性
0.1 M acetate buffer pH 5.0: soluble 10 mg/mL
运输
wet ice
储存温度
−20°C
一般描述
来自酵母的RNA在分子生物学实验室中用作核酸纯化过程中的载体。 当以10-20 μg/ml的浓度使用时，tRNA充当有效的共沉淀剂，可实现最大程度的RNA回收率（当使用有限的样品时）。
应用
来自圆拟酵母菌的核糖核酸被用作某些核糖核酸酶家族成员的核糖核酸酶活性测定的标准品。它也用作通过地衣酚测定法确定RNA浓度的标准品。
适用于核酸纯化。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
Debi Ranjan Tripathy et al.
Analytical biochemistry, 437(2), 126-129 (2013-03-19)
The ribonuclease (RNase) activity of ribonucleases has been assayed by observing the change in fluorescence intensity of ethidium bromide on binding with yeast RNA. The binding of EtBr with RNA was monitored via UV-vis and fluorimetric methods. The degradation of
Russel J Lander et al.
Biotechnology and bioengineering, 79(7), 776-784 (2002-09-05)
Preparative-scale purification of plasmid DNA has been attempted by diverse methods, including precipitation with solvents, salts, and detergents and chromatography with ion-exchange, reversed-phase, and size-exclusion columns. Chromatographic methods such as hydrophobic interaction chromatography (HIC), reversed phase chromatography (RPC), and size
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