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等级
for molecular biology
质量水平
表单
powder
质量
lyophilized
分子量
Mr ~2.5 × 106 (broad molecular weight distribution)
Mr ~2500000
包装
ampule of ≥10 units A260
储存温度
−20°C
一般描述
聚(2′-脱氧肌苷-2′-脱氧胞苷酸)钠盐是一种交替共聚物,其可作为DNA甲基转移酶的底物。
应用
聚(2′-脱氧肌苷-2′-脱氧胞苷酸) 钠盐已被用于转录因子AP-2 Alpha (TFAP2A)的电泳迁移率变动分析(EMSA),以及人胚胎肾细胞(HEK293)核提取物的Southwestern印迹中。
适用于
- EMSA中的非特异性阻断剂
- DNA甲基转移酶的评估
- DNA-小分子或药物相互作用研究
生化/生理作用
聚(2′-脱氧肌苷-2′-脱氧胞苷酸),聚(dI-dC)是一种合成的DNA底物。 在高盐下,聚(dI-dC)以左旋螺旋构象存在,并在减少盐浓度过程中恢复为右旋形式。 它在电泳迁移率变动分析(EMSA)中可用作非特异性竞争剂。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
Repeated probing of Southwestern blots using alkaline phosphatase stripping
Journal of Chromatography A, 1217(45), 7177-7181 (2010)
TFAP2A is a component of the ZEB1/2 network that regulates TGFB1-induced epithelial to mesenchymal transition
Biology Direct, 12(1), 8-8 (2017)
An enzymatic method for the determination of dCTP and dGTP in picomole amounts.
European journal of biochemistry, 17(2), 202-208 (1970-12-01)
eLife, 9 (2020-08-09)
Local activation and long-range inhibition are mechanisms conserved in self-organizing systems leading to biological patterns. A number of them involve the production by the developing cell of an inhibitory morphogen, but how this cell becomes immune to self-inhibition is rather
Nature communications, 10(1), 19-19 (2019-01-04)
Protein methyltransferases (PMTs) comprise a major class of epigenetic regulatory enzymes with therapeutic relevance. Here we present a collection of chemical probes and associated reagents and data to elucidate the function of human and murine PMTs in cellular studies. Our
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