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Merck
CN

79760

邻苯二甲醛

suitable for fluorescence, ≥99.0% (HPLC)

别名:

1,2-苯二甲醛, OPA, 邻苯二甲醛, 邻苯二醛, 酞醛

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经验公式（希尔记法）:
C8H6O2
化学文摘社编号:
643-79-8
分子量:
134.13
UNSPSC Code:
12352201
NACRES:
NA.32
PubChem Substance ID:
57652816
EC Number:
211-402-2
Beilstein/REAXYS Number:
878317
MDL number:
MFCD00003335

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产品名称

邻苯二甲醛, suitable for fluorescence, ≥99.0% (HPLC)

InChI key

ZWLUXSQADUDCSB-UHFFFAOYSA-N

InChI

1S/C8H6O2/c9-5-7-3-1-2-4-8(7)6-10/h1-6H

SMILES string

O=Cc1ccccc1C=O

assay

≥99.0% (HPLC)

form

solid

Quality Level

100

mol wt

134.13 g/mol

concentration

≤100% (o-phthaldialdehyde)

impurities

≤0.5% phthalic acid

color

white to light yellow

bp

83 °C/1.067 hPa

mp

54-56 °C

solubility

ethanol: 0.5 g/10 mL, clear, colorless to greenish-yellow
water: 37.7 g/L at 20 °C (OECD Test Guideline 105)

density

1.13 g/cm3 at 20 °C

fluorescence

λex 334 nm; λem 455 nm (Thiol Adduct)
λex 340 nm; λem 450 nm in reaction buffer (with glycine)

suitability

suitable for fluorescence

storage temp.

2-8°C

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Application

邻苯二甲醛或OPA可测定动物组织和食品样品中蛋白质中的氨基酸。对乳酸菌产生的酪胺和苯乙胺的氨基酸加合物进行定量，使用OPA作为HPLC的柱前衍生试剂。使用邻苯二甲醛作为柱前荧光剂，研究细胞内谷胱甘肽（GSH/GSSG）的形式。

Biochem/physiol Actions

苯二醛可用于高效液相色谱（HPLC）分离的氨基酸柱前衍生化。还可用于蛋白质巯基的流式细胞术测定。

General description

邻苯二甲醛，又称o-邻苯二甲醛或OPA 是一种柱前衍生化试剂。OPA与除胱氨酸外的主要氨基酸 (AA) 反应形成高荧光加合物。其衍生物的荧光激发光谱和发射光谱分别为430 nm和540 nm。HPLC技术可有效分离OPA-AA衍生物。

signalword

Danger

Hazard Classifications

Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3

target_organs

Respiratory system

存储类别

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 3

flash_point_f

269.6 °F - closed cup

flash_point_c

132 °C - closed cup

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
BCCM0599
BCCJ0409
BCCF5794
BCCC3481
BCCB5919

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José María Landete et al.
International journal of food microbiology, 115(3), 364-368 (2007-02-20)
The ability of wine lactic acid bacteria to produce tyramine and phenylethylamine was investigated by biochemical and genetic methods. An easy and accurate plate medium was developed to detect tyramine-producer strains, and a specific PCR assay that detects the presence
Zhaolai Dai et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 964, 116-127 (2014-04-16)
Studies of protein nutrition and biochemistry require reliable methods for analysis of amino acid (AA) composition in polypeptides of animal tissues and foods. Proteins are hydrolyzed by 6M HCl (110°C for 24h), 4.2M NaOH (105°C for 20 h), or proteases.
Hanne Vissenaekens et al.
Food research international (Ottawa, Ont.), 145, 110430-110430 (2021-06-12)
Flavonoid bioavailability and bioactivity is associated with interindividual variability, which is partially due to differences in health status. Previously, it was demonstrated that cellular stress, especially mitochondrial stress, increases intracellular quercetin uptake and this is associated with beneficial health effects.
Yun-Bin Han et al.
Applied microbiology and biotechnology, 99(16), 6715-6726 (2015-02-17)
Sphingolipid ceramide N-deacylase (SCDase) catalyzes reversible reactions in which the amide linkage in glycosphingolipids is hydrolyzed or synthesized. While SCDases show great value for the enzymatic synthesis of glycosphingolipids, they are relatively poorly characterized enzymes. In this work, the enzymatic
Niklas Krause et al.
Scandinavian journal of work, environment & health, 41(2), 124-139 (2015-01-20)
This study aimed to assess the effects of physically demanding work - measured as energy expenditure (EE) during occupational physical activities (OPA) - on risk of acute myocardial infarction (AMI) among men with and without preexisting ischemic heart disease (IHD).
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