等级
for fluorescence
质量水平
检测方案
≥99.0% (HPLC)
形式
solid
杂质
≤0.5% phthalic acid
mp
54-56 °C
荧光
λex 334 nm; λem 455 nm (Thiol Adduct)
λex 340 nm; λem 450 nm in reaction buffer (with glycine)
储存温度
2-8°C
SMILES字符串
O=Cc1ccccc1C=O
InChI
1S/C8H6O2/c9-5-7-3-1-2-4-8(7)6-10/h1-6H
InChI key
ZWLUXSQADUDCSB-UHFFFAOYSA-N
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一般描述
邻苯二甲醛，又称o-邻苯二甲醛或OPA 是一种柱前衍生化试剂。OPA与除胱氨酸外的主要氨基酸 (AA) 反应形成高荧光加合物。其衍生物的荧光激发光谱和发射光谱分别为430 nm和540 nm。HPLC技术可有效分离OPA-AA衍生物。
应用
邻苯二甲醛或OPA可测定动物组织和食品样品中蛋白质中的氨基酸。对乳酸菌产生的酪胺和苯乙胺的氨基酸加合物进行定量，使用OPA作为HPLC的柱前衍生试剂。使用邻苯二甲醛作为柱前荧光剂，研究细胞内谷胱甘肽（GSH/GSSG）的形式。
生化/生理作用
苯二醛可用于高效液相色谱（HPLC）分离的氨基酸柱前衍生化。还可用于蛋白质巯基的流式细胞术测定。
警示用语：
Danger
危险分类
Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3
靶器官
Respiratory system
WGK
WGK 3
闪点(°F)
closed cup
闪点(°C)
closed cup
个人防护装备
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
Hanne Vissenaekens et al.
Food research international (Ottawa, Ont.), 145, 110430-110430 (2021-06-12)
Flavonoid bioavailability and bioactivity is associated with interindividual variability, which is partially due to differences in health status. Previously, it was demonstrated that cellular stress, especially mitochondrial stress, increases intracellular quercetin uptake and this is associated with beneficial health effects.
José María Landete et al.
International journal of food microbiology, 115(3), 364-368 (2007-02-20)
The ability of wine lactic acid bacteria to produce tyramine and phenylethylamine was investigated by biochemical and genetic methods. An easy and accurate plate medium was developed to detect tyramine-producer strains, and a specific PCR assay that detects the presence
Zhaolai Dai et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 964, 116-127 (2014-04-16)
Studies of protein nutrition and biochemistry require reliable methods for analysis of amino acid (AA) composition in polypeptides of animal tissues and foods. Proteins are hydrolyzed by 6M HCl (110°C for 24h), 4.2M NaOH (105°C for 20 h), or proteases.
Yun-Bin Han et al.
Applied microbiology and biotechnology, 99(16), 6715-6726 (2015-02-17)
Sphingolipid ceramide N-deacylase (SCDase) catalyzes reversible reactions in which the amide linkage in glycosphingolipids is hydrolyzed or synthesized. While SCDases show great value for the enzymatic synthesis of glycosphingolipids, they are relatively poorly characterized enzymes. In this work, the enzymatic
I Molnár-Perl
Journal of chromatography. A, 913(1-2), 283-302 (2001-05-18)
An overview is presented of HPLC methods currently in use to determine amino acids as their o-phthaldialdyde derivatives in the presence of various SH-group-containing additives. Crucial points that proved to influence the stability of the amino acid OPA derivatives have
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