79390
磷酸酶(碱性) 来源于小牛肠粘膜
suitable for enzyme immunoassay, solution (clear, colorless), ~2500 U/mg protein (~10 mg/ml)
别名:
Alkaline Phosphatase from calf intestinal mucosa
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化学文摘社编号:
UNSPSC Code:
12352204
eCl@ss:
42010105
EC Number:
232-631-4
NACRES:
NA.54
MDL number:
Specific activity:
~2500 U/mg protein (~10 mg/ml)
Biological source:
bovine (calf) intestinal mucosa
产品名称
磷酸酶(碱性) 来源于小牛肠粘膜, suitable for enzyme immunoassay, solution (clear, colorless), ~2500 U/mg protein (~10 mg/ml)
biological source
bovine (calf) intestinal mucosa
form
solution (clear, colorless)
specific activity
~2500 U/mg protein (~10 mg/ml)
mol wt
Mr ~140000
technique(s)
enzyme immunoassay: suitable
impurities
0.1 mM ZnCl2
1 mM MgCl2
3 M NaCl
30 mM triethanolamine
pH
~7.6
storage temp.
2-8°C
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Other Notes
1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol 4-nitrophenyl phosphate per minute at pH 9.8 and 37 °C
Sales restrictions may apply
Used in highly sensitive (pg/m) enzyme immunoassays with LCEC detection of phenol; Comparison of three methods for conjugating alkaline phosphatase to human placental lactogen; Preparation of enzyme-antibody conjugates; Liposome immunoassay with antigen-coupled liposomes containing alkaline phosphatase
Application
Alkaline phosphatase is used in the laboratory to remove phosphate monoesters which prevents self-ligation. It is also used in the dairy industry to confirm pasturization of milk. Alkaline phosphatse is commonly conjugated to antibodies and used in immunoblotting, ELISA′s, and other antibody based detection assays.
Biochem/physiol Actions
Alkaline phosphatase is a hydrolytic enzyme which dephosphorylates a variety of molecules under basic conditions.
Preparation Note
The solution can be used without dialysis (for enzyme immuno-assay).
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
动植物源性产品
此项目有
M.I. Halliday et al.
Biochemical Society Transactions, 14, 473-473 (1986)
D G Williams
Journal of immunological methods, 72(1), 261-268 (1984-08-03)
One of the requirements for enzyme immunoassay is the formation of a labelled component of the assay system which can be either the ligand or the binder. Three methods for conjugating alkaline phosphatase to human placental lactogen were investigated, involving
K.R. Wehrmeyer et al.
Journal of Liquid Chromatography, 6, 2141-2141 (1983)
Opioid receptor agonists enhance the phosphorylation state of Fas-associated death domain (FADD) protein in the rat brain: functional interactions with casein kinase Ialpha, Galpha(i) proteins, and ERK1/2 signaling.
Garcia-Fuster, M.J., et al.
Neuropsychopharmacology, 55, 886-899 (2008)
C K Kim et al.
Journal of immunological methods, 159(1-2), 101-106 (1993-02-26)
Immunoliposomes were prepared and their immunoassay applications investigated. Liposomes were prepared from cholesterol and phospholipids including maleimidobenzoylphosphatidylethanolamine (MBPE) for conjunction with thiol-containing antigens. Alkaline phosphatase (APase) was entrapped in the liposome and BSA, the antigen, was modified by reaction with
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