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此商品 | P6774 | A2356 | P5521 |
|---|---|---|---|
| technique(s) enzyme immunoassay: suitable | technique(s) - | technique(s) - | technique(s) - |
| biological source bovine (calf) intestinal mucosa | biological source bovine intestinal mucosa | biological source - | biological source - |
| specific activity ~2500 U/mg protein (~10 mg/ml) | specific activity ≥2,000 DEA units/mg protein | specific activity ≥5,500 DEA units/mg protein | specific activity ≥2,000 DEA units/mg protein |
| form solution (clear, colorless) | form buffered aqueous solution | form (Solution in 40% glycerol containing 6 mM Tris, 6 mM MgCl2 and 0.12 mM ZnCl2, pH approximately 7.6) | form aqueous solution |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| pH ~7.6 | pH - | pH - | pH - |
应用
Alkaline phosphatase is used in the laboratory to remove phosphate monoesters which prevents self-ligation. It is also used in the dairy industry to confirm pasturization of milk. Alkaline phosphatse is commonly conjugated to antibodies and used in immunoblotting, ELISA′s, and other antibody based detection assays.
生化/生理作用
Alkaline phosphatase is a hydrolytic enzyme which dephosphorylates a variety of molecules under basic conditions.
单位定义
1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol 4-nitrophenyl phosphate per minute at pH 9.8 and 37 °C
重悬
The solution can be used without dialysis (for enzyme immuno-assay).
其他说明
Sales restrictions may apply
Used in highly sensitive (pg/m) enzyme immunoassays with LCEC detection of phenol[1]; Comparison of three methods for conjugating alkaline phosphatase to human placental lactogen[2]; Preparation of enzyme-antibody conjugates[3][4]; Liposome immunoassay with antigen-coupled liposomes containing alkaline phosphatase[5]
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
动植物源性产品
Opioid receptor agonists enhance the phosphorylation state of Fas-associated death domain (FADD) protein in the rat brain: functional interactions with casein kinase Ialpha, Galpha(i) proteins, and ERK1/2 signaling.
Garcia-Fuster, M.J., et al.
Neuropsychopharmacology, 55, 886-899 (2008)
Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes.
E Engvall et al.
Journal of immunology (Baltimore, Md. : 1950), 109(1), 129-135 (1972-07-01)
C K Kim et al.
Journal of immunological methods, 159(1-2), 101-106 (1993-02-26)
Immunoliposomes were prepared and their immunoassay applications investigated. Liposomes were prepared from cholesterol and phospholipids including maleimidobenzoylphosphatidylethanolamine (MBPE) for conjunction with thiol-containing antigens. Alkaline phosphatase (APase) was entrapped in the liposome and BSA, the antigen, was modified by reaction with
M.I. Halliday et al.
Biochemical Society Transactions, 14, 473-473 (1986)
D G Williams
Journal of immunological methods, 72(1), 261-268 (1984-08-03)
One of the requirements for enzyme immunoassay is the formation of a labelled component of the assay system which can be either the ligand or the binder. Three methods for conjugating alkaline phosphatase to human placental lactogen were investigated, involving
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