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表单
solid
溶解性
DMF: 0.25 mg/mL, clear
荧光
λex 590 nm; λem 600-620 nm in PBS, pH 7
储存温度
−20°C
一般描述
Abberior CAGE 590 is a masked dye which is initially colorless and nonfluorescent. When illuminated with UV light it undergoes a rapid uncaging reaction and releases a highly fluorescent dye. The dye opens up the red fluorescent regime for CAGE dyes. It can most effectively be excited from 560 to 600nm.
Abberior CAGE 590 is designed for single-molecule photoswitching microscopy modes such as PALM, STORM and GSDIM and, in its uncaged form, also performs well in STED microscopy. Further, after photoactivation, Abberior CAGE markers can be tracked in confocal and epifluorescence microscopy to analyse molecular dynamics such as diffusion, flow directions and velocities, e.g. are tracking experiments in which the fluorescence signal is observed over time after the initial release of the fluorophore.
Key Features
Absorption Maximum, λmax: 262, 325, 351 nm (non-activated, PBS, pH 7.4)
595 nm (photoactivated, PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 75,000 M-1cm-1 (photoactivated, PBS, pH 7.4)
Fluorescence Maximum, λfl: 615 nm (photoactivated, PBS, pH 7.4)
Photoactivation Wavelength:360 - 440 nm
Recommended STED Wavelength, λSTED: 685 −715 nm
Fluorescence Quantum Yield, η: 0.80 (after photoactivation, PBS, pH 7.47)
Abberior CAGE 590 is designed for single-molecule photoswitching microscopy modes such as PALM, STORM and GSDIM and, in its uncaged form, also performs well in STED microscopy. Further, after photoactivation, Abberior CAGE markers can be tracked in confocal and epifluorescence microscopy to analyse molecular dynamics such as diffusion, flow directions and velocities, e.g. are tracking experiments in which the fluorescence signal is observed over time after the initial release of the fluorophore.
Key Features
- First CAGE dye in the red fluorescent range
- Ideal for PALM, STORM, GSDIM
- Well suited as 2nd STED color (after uncaging)
Absorption Maximum, λmax: 262, 325, 351 nm (non-activated, PBS, pH 7.4)
595 nm (photoactivated, PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 75,000 M-1cm-1 (photoactivated, PBS, pH 7.4)
Fluorescence Maximum, λfl: 615 nm (photoactivated, PBS, pH 7.4)
Photoactivation Wavelength:360 - 440 nm
Recommended STED Wavelength, λSTED: 685 −715 nm
Fluorescence Quantum Yield, η: 0.80 (after photoactivation, PBS, pH 7.47)
应用
Abberior® CAGE 590 anti-rabbit antibody has been used as a secondary antibody for spectral-FPALM (fluorescence photo-activation localization microscopy) imaging of NIH-3T3 cells.
适用性
Designed and tested for fluorescent super-resolution microscopy
分析说明
degree of coupling: report results
其他说明
For more information on Abberior® Dyes, please visit our overview.
法律信息
abberior is a registered trademark of Abberior GmbH
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
Michael J Mlodzianoski et al.
PloS one, 11(3), e0147506-e0147506 (2016-03-24)
Localization microscopy can image nanoscale cellular details. To address biological questions, the ability to distinguish multiple molecular species simultaneously is invaluable. Here, we present a new version of fluorescence photoactivation localization microscopy (FPALM) which detects the emission spectrum of each
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