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一般描述
青霉素酰胺酶是具有A链和B链（分别为209和566个氨基酸）的周质80K异二聚体。它广泛分布于微生物中，包括细菌、酵母和丝状真菌。在所有来源中，对来自大肠杆菌的酶表征最充分，并且常用于工业应用。
应用
青霉素酰胺酶被用于研究其在抗生素降解中对AHL（N-酰基高丝氨酸内酯）释放脂肪酸和HSL（高丝氨酸内酯）的影响。 在乳酸杆菌中胆汁盐水解酶和青霉素酰基转移酶家族成员的功能分析研究中，它被用作青霉素G酰基转移酶活性检测的阳性对照。 青霉素酰胺酶可用于从青霉素-G合成6-氨基青霉烷酸以及用于β-内酰胺抗生素的工业生产。
生化/生理作用
通过疏水性蛋白质层析在大肠杆菌中生物合成青霉素酰胺酶是一个由代谢碳源（如多元醇、羧酸等）调节的诱导反应。它还受到分解代谢物抑制的影响。 它通过酰基-酶中间体催化酰胺键的形成。
单位定义
1个酶活性单位是指在pH 7.6、37°C条件下，每分钟水解1 μmol苄青霉素所需的酶量
其他说明
表征；对映选择性分离；氨苄西林和苄青霉素的合成
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Penicillin Acylase in the Industrial Production of ?-Lactam Antibiotics
Bruggink A, Roos EC, Vroom ED
Organic Process Research & Development, 2(2), 128-133 (1998)
Penicillin acylase (bacterial).
T A Savidge et al.
Methods in enzymology, 43, 705-721 (1975-01-01)
V Kasche et al.
Hoppe-Seyler's Zeitschrift fur physiologische Chemie, 365(12), 1435-1443 (1984-12-01)
Hydrophobic protein chromatography was used to prepare homogeneous fractions of penicillin amidase (EC 3.5.1.11) from E. coli. The apparent ratios of the rate constants for the deacylation of the acyl-penicillin amidase formed in the hydrolysis of phenylacetylglycine or D-phenylglycine methyl
Yi-Han Lin et al.
Molecular microbiology, 47(3), 849-860 (2003-01-22)
N-acylhomoserine lactones (AHLs) are used as signal molecules by many quorum-sensing Proteobacteria. Diverse plant and animal pathogens use AHLs to regulate infection and virulence functions. These signals are subject to biological inactivation by AHL-lactonases and AHL-acylases. Previously, little was known
Pedro Valencia et al.
New biotechnology, 29(2), 218-226 (2011-09-20)
A mathematical model is presented for the kinetically controlled synthesis of cephalexin that describes the heterogeneous reaction-diffusion process involved in a batch reactor with glyoxyl-agarose immobilized penicillin acylase. The model is based on equations considering reaction and diffusion components. Reaction
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