一般描述
木瓜蛋白酶是一种天然存在于木瓜(Carica papaya L.)中的蛋白水解酶。可以从切开的未成熟木瓜的乳胶中获得。木瓜蛋白酶属于半胱氨酸蛋白酶家族。它是一种球状单链蛋白质,由四个二硫桥和催化基础残基组成。木瓜蛋白酶由全α-结构域和反平行片β-结构域组成。
应用
从番木瓜提取的木瓜蛋白酶已用于:
- 酶解大鼠皮质以提取用于细胞培养的星形胶质细胞
- 研究对羊驼精浆粘度和精子功能的影响
- 研究对犀牛精子产量和低温存活率的影响
木瓜蛋白酶被用作嫩肉精。它还被用于在原代细胞培养物制备中分解组织。
生化/生理作用
木瓜蛋白酶对许多生物体的生物过程起重要作用。它对蛋白质、氨基酸酯和酰胺连接以及短链肽具有蛋白水解活性。木瓜蛋白酶倾向于切割碱性氨基酸如精氨酸、苯丙氨酸和赖氨酸的肽键。它在药物发现、制药、食品、烘焙和乳制品行业有广泛的应用。
木瓜蛋白酶是一种可切割肽键的半胱氨酸蛋白酶,并且已知可切割抗体的Fc部分。
单位定义
1 U对应于在pH 6.2和25°C下,每分钟水解1 μmol N-苯甲酰基-L-精氨酸乙酯(BAEE,货号 12880)所需的酶量
警示用语:
Danger
危险分类
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
法规信息
动植物源性产品
Extraction of Near-Field Fluorescence from Composite Signals to Provide High Resolution Images of Glial Cells
Biophysical Journal, 80(5), 2477-2482 (2001)
The effect of glycosaminoglycan enzymes and proteases on the viscosity of alpaca seminal plasma and sperm function
Animal Reproduction Science, 138(1-2), 261-267 (2013)
Immobilization of papain: A review
International Journal of Biological Macromolecules, 188, 94-113 (2021)
Journal of food science, 74(6), H168-H173 (2009-09-03)
This study aims to understand the enhancing effect of glycosaminoglycans (GAGs), such as chondroitin/dermatan structures, on Fe uptake to Caco-2 cells. High-sulfated GAGs were selectively purified from cooked haddock. An in vitro digestion/Caco-2 cell culture model was used to evaluate
Journal of agricultural and food chemistry, 56(21), 10346-10351 (2008-10-15)
This study continues previous research to confirm that glycosaminoglycans (GAGs) exert a positive effect on promoting iron uptake by Caco-2 cells. Cooked haddock was digested with papain, and GAGs were further purified on the basis of their sulfur content. Reverse
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