推荐产品
方案
≥95% (UV)
表单
powder
溶解性
methanol: soluble
荧光
λex 523 nm; λem 630 nm in 0.1 M Tris pH 9.0
适用性
suitable for fluorescence
运输
wet ice
储存温度
−20°C
SMILES字符串
CCCC1=NOC(=O)\C1=C/C=C/C=C/c2c(O)onc2CCC
InChI
1S/C17H20N2O4/c1-3-8-14-12(16(20)22-18-14)10-6-5-7-11-13-15(9-4-2)19-23-17(13)21/h5-7,10-11,20H,3-4,8-9H2,1-2H3/b7-5+,10-6+,13-11-
InChI key
CPHARWHMAGUXDW-PQWGHOHWSA-N
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应用
Oxonol VI can be used as a fluorescent potentiometric indicator to detect the membrane potentials in lipid vesicles. Quantitative measurements of the membrane potentials can be easily calibrated using oxonol VI because of its response to membrane potential changes. It is also used as an electric potential-sensitive probe to study membrane vesicles from E. coli cells.
包装
Bottomless glass bottle. Contents are inside inserted fused cone.
其他说明
As an optical indicator for membrane potentials in lipid vesicles
储存分类代码
13 - Non Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
Selective electrodiffusion of zinc ions in a Zrt-, Irt-like protein, ZIPB
Journal of biological and chemical chronicles, 285(50), 39013-39020 (2010)
Preparation of Everted Membrane Vesicles from Escherichia coli Cells
Bio-protocol, 7(9), e2254-e2254 (2017)
Ratiometric fluorescence measurements of membrane potential generated by yeast plasma membrane H+-ATPase reconstituted into vesicles
Biochimica et Biophysica Acta - Biomembranes, 1609(1), 71-79 (2003)
Journal of biochemistry, 121(4), 746-755 (1997-04-01)
To investigate the energy-conserving function of the NADH:ubiquinone reductase (complex I), we have selected oxonol VI [bis(3-propyl-5-oxoisoxazol-4-yl)pentamethine oxonol] as the most sensitive probe for measuring the reactions of membrane potential generation in submitochondrial particles. Calibration of the oxonol signals with
Membrane biochemistry, 5(3), 225-241 (1984-01-01)
Beef heart mitochondrial H+-ATPase (F1-F0) vesicles were prepared by lysolecithin extraction of ETPH. ATP-driven membrane potential was monitored indirectly by following absorbance changes of the potential-sensitive dye oxonol VI. The steady-state potential was discharged by oligomycin and/or Cd2+ (a dithiol
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