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Merck
CN

75926

Sigma-Aldrich

Oxonol VI

suitable for fluorescence, ≥95% (UV)

别名:

1,5-Bis(5-oxo-3-propylisoxazol-4-yl)pentamethine oxonol

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About This Item

经验公式(希尔记法):
C17H20N2O4
CAS号:
分子量:
316.35
MDL编号:
UNSPSC代码:
12171500
PubChem化学物质编号:
NACRES:
NA.32

方案

≥95% (UV)

表单

powder

溶解性

methanol: soluble

荧光

λex 523 nm; λem 630 nm in 0.1 M Tris pH 9.0

适用性

suitable for fluorescence

运输

wet ice

储存温度

−20°C

SMILES字符串

CCCC1=NOC(=O)\C1=C/C=C/C=C/c2c(O)onc2CCC

InChI

1S/C17H20N2O4/c1-3-8-14-12(16(20)22-18-14)10-6-5-7-11-13-15(9-4-2)19-23-17(13)21/h5-7,10-11,20H,3-4,8-9H2,1-2H3/b7-5+,10-6+,13-11-

InChI key

CPHARWHMAGUXDW-PQWGHOHWSA-N

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应用

Oxonol VI can be used as a fluorescent potentiometric indicator to detect the membrane potentials in lipid vesicles. Quantitative measurements of the membrane potentials can be easily calibrated using oxonol VI because of its response to membrane potential changes. It is also used as an electric potential-sensitive probe to study membrane vesicles from E. coli cells.

包装

Bottomless glass bottle. Contents are inside inserted fused cone.

其他说明

As an optical indicator for membrane potentials in lipid vesicles

储存分类代码

13 - Non Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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访问文档库

Selective electrodiffusion of zinc ions in a Zrt-, Irt-like protein, ZIPB
Lin W, et al.
Journal of biological and chemical chronicles, 285(50), 39013-39020 (2010)
Preparation of Everted Membrane Vesicles from Escherichia coli Cells
Verkhovskaya M
Bio-protocol, 7(9), e2254-e2254 (2017)
Ratiometric fluorescence measurements of membrane potential generated by yeast plasma membrane H+-ATPase reconstituted into vesicles
Holoubek A, et al.
Biochimica et Biophysica Acta - Biomembranes, 1609(1), 71-79 (2003)
A Ghelli et al.
Journal of biochemistry, 121(4), 746-755 (1997-04-01)
To investigate the energy-conserving function of the NADH:ubiquinone reductase (complex I), we have selected oxonol VI [bis(3-propyl-5-oxoisoxazol-4-yl)pentamethine oxonol] as the most sensitive probe for measuring the reactions of membrane potential generation in submitochondrial particles. Calibration of the oxonol signals with
M J Pringle et al.
Membrane biochemistry, 5(3), 225-241 (1984-01-01)
Beef heart mitochondrial H+-ATPase (F1-F0) vesicles were prepared by lysolecithin extraction of ETPH. ATP-driven membrane potential was monitored indirectly by following absorbance changes of the potential-sensitive dye oxonol VI. The steady-state potential was discharged by oligomycin and/or Cd2+ (a dithiol

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