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Merck
CN

75784

Sigma-Aldrich

Atto 565

BioReagent, suitable for fluorescence

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经验公式(希尔记法):
C31H31ClN2O9
分子量:
611.04
MDL编号:
UNSPSC代码:
12352200
NACRES:
NA.32

产品线

BioReagent

质量水平

检测方案

≥90.0% (HPLC)

形式

powder

制造商/商品名称

ATTO-TEC GmbH

λ

in ethanol (with 0.1% trifluoroacetic acid)

紫外吸收

λ: 562.0-568.0 nm Amax

适用性

suitable for fluorescence

储存温度

−20°C

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应用

Atto 荧光标记物设计用于高灵敏度应用,包括单分子检测。Atto 标记具有刚性结构,未显示任何顺反异构化。因此,这些标记物在偶联时显示出极低的光谱偏移。
Atto 565 在水中摩尔消光为 120.000,在含 97% 乙醇的水溶液中 QY 为 92%。衰变时间是 3.4 ns。
组氨酸标记蛋白的特异稳定荧光标记

法律信息

本产品仅供研究使用。如果打算商业化,请联系知识产权持有者(德国ATTO-TEC GmbH公司)申请许可。

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


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Suman Lata et al.
Journal of the American Chemical Society, 128(7), 2365-2372 (2006-02-16)
Labeling of proteins with fluorescent dyes offers powerful means for monitoring protein interactions in vitro and in live cells. Only a few techniques for noncovalent fluorescence labeling with well-defined localization of the attached dye are currently available. Here, we present
Takao Nakata et al.
The Journal of cell biology, 194(2), 245-255 (2011-07-20)
Polarized transport in neurons is fundamental for the formation of neuronal circuitry. A motor domain-containing truncated KIF5 (a kinesin-1) recognizes axonal microtubules, which are enriched in EB1 binding sites, and selectively accumulates at the tips of axons. However, it remains
Łukasz Krzemiński et al.
Journal of the American Chemical Society, 133(38), 15085-15093 (2011-08-26)
A combined fluorescence and electrochemical method is described that is used to simultaneously monitor the type-1 copper oxidation state and the nitrite turnover rate of a nitrite reductase (NiR) from Alcaligenes faecalis S-6. The catalytic activity of NiR is measured
PALM and STORM. Unlocking live-cell super-resolution.
Henriques, R.; Griffiths, C.; Hesper Rego, E.; Mhlanga, Musa M.
Biopolymers, 95(5), 322-331 (2011)
Direct stochastic optical reconstruction microscopy with standard fluorescent probes.
van de Linde, S.; et al.
Nature Protocols, 6(7), 991-1009 (2011)

商品

Fluorescence lifetime measurement is advantageous over intensity-based measurements. Applications include fluorescence lifetime assays, sensing and FLI.

荧光寿命测量与基于强度的测量相比是有优势的。其应用包括荧光寿命分析,感测和FLI。

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