产品线
BioReagent
质量水平
检测方案
≥90% (HPLC)
≥90% (degree of coupling)
形式
powder
制造商/商品名称
ATTO-TEC GmbH
适用性
suitable for fluorescence
检测方法
fluorometric
储存温度
−20°C
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一般描述
Atto 565 NHS 脂是一种常用的胺反应试剂，由 ATTO-TEC 提供。它广泛用于蛋白质标记，且易于与含氨基的化合物（如蛋白质）反应，以在染料之间形成化学稳定的酰胺键，。NHS 脂偶联最佳 pH 值范围为 pH 8.0 - 9.0。在该 pH 值下，蛋白质的氨基基团（比如赖氨酸的 ε-氨基基团）非质子化至足够高以快速耦合。
特点和优势
- 强吸收力
- 高荧光量子产率
- 高热稳定性和光稳定性
其他说明
生物结合物的荧光 ；用于荧光共振能量转移，FRET，以及各种实验 。
法律信息
本产品仅供研究使用。如果打算商业化，请联系知识产权持有者（德国ATTO-TEC GmbH公司）申请许可。
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
Michael Cooper et al.
Journal of fluorescence, 14(2), 145-150 (2004-12-24)
The spectral properties of a rigidified trimethine cyanine dye, Cy3B have been characterised. This probe has excellent fluorescent properties, good water solubility and can be bioconjugated. The emission properties of this fluorophore have also been investigated upon conjugation to an
Benjamin Zoller et al.
Cell, 175(3), 835-847 (2018-10-20)
How transcriptional bursting relates to gene regulation is a central question that has persisted for more than a decade. Here, we measure nascent transcriptional activity in early Drosophila embryos and characterize the variability in absolute activity levels across expression boundaries.
Elizabeth A Jares-Erijman et al.
Nature biotechnology, 21(11), 1387-1395 (2003-11-05)
Förster (or Fluorescence) Resonance Energy Transfer (FRET) is unique in generating fluorescence signals sensitive to molecular conformation, association, and separation in the 1-10 nm range. We introduce a revised photophysical framework for the phenomenon and provide a systematic catalog of
Rachael Bakker et al.
eLife, 9 (2020-06-23)
Morphogen signaling contributes to the patterned spatiotemporal expression of genes during development. One mode of regulation of signaling-responsive genes is at the level of transcription. Single-cell quantitative studies of transcription have revealed that transcription occurs intermittently, in bursts. Although the
Matthew G Niepielko et al.
Current biology : CB, 28(12), 1872-1881 (2018-06-05)
The formation of ribonucleoprotein assemblies called germ granules is a conserved feature of germline development. In Drosophila, germ granules form at the posterior of the oocyte in a specialized cytoplasm called the germ plasm, which specifies germline fate during embryogenesis. mRNAs
商品
Chromogenic and fluorogenic derivatives are invaluable tools for biochemistry, having numerous applications in enzymology, protein chemistry, immunology and histochemistry.
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