71028
单溴（三甲基铵）二溴化溴
suitable for fluorescence, ≥90.0% (HPLC)
别名:
5-(溴甲基) -N,N,N,2,6-五甲基-1,7-二氧代-1H,7H-吡唑并[1,2-A]吡唑-3-甲基溴化铵, Brombimane q, qBBr, 硫醇MQ, （三甲基铵）单溴二烯
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关于此项目
经验公式（希尔记法）:
C13H19Br2N3O2
化学文摘社编号:
分子量:
409.12
UNSPSC Code:
12171500
NACRES:
NA.32
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
4630658
产品名称
单溴（三甲基铵）二溴化溴, suitable for fluorescence, ≥90.0% (HPLC)
InChI key
FCRPMMVPBVOKQS-UHFFFAOYSA-M
SMILES string
[Br-].CC1=C(CBr)N2N(C1=O)C(=O)C(C)=C2C[N+](C)(C)C
InChI
1S/C13H19BrN3O2.BrH/c1-8-10(6-14)15-11(7-17(3,4)5)9(2)13(19)16(15)12(8)18;/h6-7H2,1-5H3;1H/q+1;/p-1
assay
≥90.0% (HPLC)
form
powder
solubility
DMSO: 5 mg/mL
fluorescence
λex 380 nm; λem 475 nm in 0.1 M phosphate pH 7.5
suitability
suitable for fluorescence
storage temp.
2-8°C
Quality Level
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Biochem/physiol Actions
在与低分子量硫醇发生共轭反应之前，单溴二胺基本上是不发荧光的。与普通的单溴二胺不同，它带有正电荷，因此可以通过电泳或阳离子交换色谱法分离缀合物
Other Notes
用于标记生物材料中硫醇的试剂
Packaging
无底玻璃瓶。内含物在插入的融合锥体内。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
S X Yuan et al.
FEBS letters, 234(1), 218-222 (1988-07-04)
The single cysteinyl residue 26 of spinach calmodulin was labelled with the thiol-specific bimane fluorescence probe. Following application of stoichiometric quantities of Ca2+ or aluminum ions to the protein, temperature-dependent fluorescence changes (anisotropy, lifetime) could be monitored via the label.
Conformational changes in mitochondrial aspartate aminotransferase detected by a covalently attached fluorescent probe.
E Sandmeier et al.
Progress in clinical and biological research, 144B, 117-124 (1984-01-01)
Fluorescent localization of thiols and disulfides in marsupial spermatozoa by bromobimane labelling.
K E Mate et al.
Molecular reproduction and development, 37(3), 318-325 (1994-03-01)
The acrosome of marsupial spermatozoa is a robust structure which, unlike its placental counterpart, resists disruption by detergent or freeze/thawing and does not undergo a calcium ionophore induced acrosome reaction. In this study specific fluorescent thiol labels, bromobimanes, were used
T T Huang et al.
Biology of reproduction, 31(4), 797-809 (1984-11-01)
The distribution of thiols and disulfides in the guinea pig spermatozoon during maturation and capacitation was studied using both membrane-permeable (mBBr) and impermeable (qBBr) forms of bromobimane, a specific fluorescent probe for thiol groups. In conjunction with the disulfide (SS)-reducing
M Balázs et al.
Biochemical and biophysical research communications, 140(3), 999-1006 (1986-11-14)
The accessibility of cell surface sulfhydryl groups in human peripheral lymphocytes was investigated with 5,5'-dithiobis-(2-nitrobenzoic acid) in the presence and absence of ionophore antibiotics and the monoclonal antibody, OKT-3. Only a few accessible protein thiols have been found on the
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