70540
萘酚黄 S
suitable for microscopy
别名:
2,4-二硝基-1-萘酚-7-磺酸 钠盐, 8-羟基-5,7-二硝基-2-萘磺酸 钠盐, 酸性黄 1, 黄胺酸 钠盐
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经验公式（希尔记法）:
C10H4N2Na2O8S
化学文摘社编号:
分子量:
358.19
EC Number:
212-690-2
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
MDL number:
Colour Index Number:
10316
Beilstein/REAXYS Number:
3839220
InChI key
CTIQLGJVGNGFEW-UHFFFAOYSA-L
InChI
1S/C10H6N2O8S.2Na/c13-10-7-3-5(21(18,19)20)1-2-6(7)8(11(14)15)4-9(10)12(16)17;;/h1-4,13H,(H,18,19,20);;/q;2*+1/p-2
SMILES string
[Na+].[Na+].[O-]c1c(cc([N+]([O-])=O)c2ccc(cc12)S([O-])(=O)=O)[N+]([O-])=O
form
powder
solubility
methanol: water (1:1): 0.5 g/10 mL, clear
εmax
≥275 at 423-433 nm in water, ≥≥ 250 at 387-397 nm in water
suitability
suitable for microscopy
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
Quality Level
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Application
Naphthol yellow S has been used as a staining agent in fixed human embryonic stem cell-derived cardiomyocytes.
Biochem/physiol Actions
Naphthol yellow S (NYS) is used as a stain for protein basic groups. It is an acidic dye and forms a NYS-protein complex at acidic pH. Combination of feulgen and NYS provides DNA to protein ratio.
Analysis Note
λmax. ∼428 nm/∼392 nm; E(1%,1 cm) ∼275-425/∼250-400 (H2O)
signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1 - STOT RE 2
存储类别
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Scholtissek C, et al.
Chemistry and Cytochemistry of Nucleic Acids and Nuclear Proteins (2012)
J James et al.
Cell and tissue research, 243(1), 165-169 (1986-01-01)
With regard to the protein content, as analysed cytophotometrically, of hepatocytes from rats kept under a 12L 12D photoperiod (photophase 7:00-19:00), the following facts have been established: 1) Hepatocytes of different classes of ploidy all demonstrate, more or less equally
Cellular dehydrogenase assay with a tridensity-automicrophotometer.
M Yamada et al.
Cellular and molecular biology, 29(3), 261-266 (1983-01-01)
Brodsky VYa et al.
European journal of histochemistry : EJH, 37(3), 199-206 (1993-01-01)
Two-wavelength scanning cytophotometry was performed for the measurement of DNA (Feulgen reaction) and total protein content (naphthol yellow S staining) in the same cardiac myocyte. A lack of proportionality between the DNA content (i.e, cell ploidy) and protein content has
M Iwatsuru et al.
Journal of pharmacobio-dynamics, 4(11), 851-859 (1981-11-01)
The characteristics of the binding site in the first binding class of naphthol yellow-S (NY-S) on bovine serum albumin (BSA) were studied. The binding of NY-S to BSA at an equimolar ratio of each material resulted in a marked quenching
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