70540
萘酚黄 S
for microscopy (Hist.), for the precipitation (of amino acids and peptides)
别名:
2,4-二硝基-1-萘酚-7-磺酸 钠盐, 8-羟基-5,7-二硝基-2-萘磺酸 钠盐, 酸性黄 1, 黄胺酸 钠盐
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所有图片(1)
About This Item
经验公式（希尔记法）:
C10H4N2Na2O8S
CAS号:
分子量:
358.19
颜色索引号:
10316
Beilstein:
3839220
EC 号:
MDL编号:
UNSPSC代码:
12171500
PubChem化学物质编号:
NACRES:
NA.47
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等级
for microscopy (Hist.)
for the precipitation (of amino acids and peptides)
质量水平
表单
powder
溶解性
methanol: water (1:1): 0.5 g/10 mL, clear
εmax
≥275 at 423-433 nm in water
≥≥ 250 at 387-397 nm in water
应用
diagnostic assay manufacturing
hematology
histology
储存温度
room temp
SMILES字符串
[Na+].[Na+].[O-]c1c(cc([N+]([O-])=O)c2ccc(cc12)S([O-])(=O)=O)[N+]([O-])=O
InChI
1S/C10H6N2O8S.2Na/c13-10-7-3-5(21(18,19)20)1-2-6(7)8(11(14)15)4-9(10)12(16)17;;/h1-4,13H,(H,18,19,20);;/q;2*+1/p-2
InChI key
CTIQLGJVGNGFEW-UHFFFAOYSA-L
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应用
Naphthol yellow S has been used as a staining agent in fixed human embryonic stem cell-derived cardiomyocytes.
生化/生理作用
Naphthol yellow S (NYS) is used as a stain for protein basic groups. It is an acidic dye and forms a NYS-protein complex at acidic pH. Combination of feulgen and NYS provides DNA to protein ratio.
分析说明
λmax. ∼428 nm/∼392 nm; E(1%,1 cm) ∼275-425/∼250-400 (H2O)
警示用语：
Warning
危险声明
危险分类
Skin Sens. 1 - STOT RE 2
储存分类代码
11 - Combustible Solids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
从最新的版本中选择一种：
Scholtissek C, et al.
Chemistry and Cytochemistry of Nucleic Acids and Nuclear Proteins (2012)
W M Frederiks et al.
Histochemistry, 68(1), 49-53 (1980-01-01)
The purpose of this study is to compare the protein content of parenchymal and non-parenchymal nuclei, as isolated from rat liver. The nuclei have been separated by means of a 1 g-sedimentation technique. The protein content of the separated nuclei
J Tas et al.
Acta histochemica. Supplementband, 20, 69-73 (1979-01-01)
After staining with Naphthol Yellow S (NYS) at optimal conditions of pH (2.8), the protein content of rat liver cells isolated by means of a collagenase perfusion technique was found to be cytophotometrically immeasurable, because of too high local dye
Brodsky VYa et al.
European journal of histochemistry : EJH, 37(3), 199-206 (1993-01-01)
Two-wavelength scanning cytophotometry was performed for the measurement of DNA (Feulgen reaction) and total protein content (naphthol yellow S staining) in the same cardiac myocyte. A lack of proportionality between the DNA content (i.e, cell ploidy) and protein content has
N W Schipper et al.
Laboratory investigation; a journal of technical methods and pathology, 61(2), 228-234 (1989-08-01)
The paper describes an improved segmentation method to measure the percentages of epithelium and stroma in ovarian (tumor) tissue with automated image analysis and evaluates its prognostic value. In the image processing method, a blue-yellow image pair is recorded from
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