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69898

Sigma-Aldrich

溴乙烷

suitable for fluorescence, BioReagent, ≥95% (HPCE)

别名:

单溴代二苯甲烷

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About This Item

经验公式（希尔记法）:
C10H11BrN2O2
CAS号:
71418-44-5
分子量:
271.11
Beilstein:
4430959
MDL编号:
MFCD00036951
UNSPSC代码:
12171500
PubChem化学物质编号:
57652223
NACRES:
NA.32

产品线

BioReagent

质量水平

100

方案

≥95% (HPCE)

表单

solid

mp

152-154 °C (lit.)

溶解性

DMF: soluble
DMSO: soluble
acetonitrile: soluble
methanol: soluble

荧光

λex 390 nm; λem 478 nm in 0.1 M phosphate pH 7.5 (after derivatization with glutathione)
λex 398 nm

适用性

suitable for fluorescence

储存温度

2-8°C

SMILES字符串

CC1=C(C)C(=O)N2N1C(CBr)=C(C)C2=O

InChI

1S/C10H11BrN2O2/c1-5-7(3)12-8(4-11)6(2)10(15)13(12)9(5)14/h4H2,1-3H3

InChI key

AHEWZZJEDQVLOP-UHFFFAOYSA-N

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相关类别

一般描述

溴乙烷也被称为硫醇探针，是一种通过光解反应激活的荧光试剂。溴乙烷在溶液中与小硫醇基团（比如 GSH）和有反应活性的蛋白硫醇基团（如 血红蛋白）反应。 溴乙烷与硫醇的反应是二级反应，和pH相关。并且，通过与硫醇反应，激活了用于检测的水溶性荧光物质。

应用

溴乙烷被用于通过HPLC测定硫醇。 它适用于作为荧光测定2,3-二巯基丙烷-1-磺酸和其他二硫醇的柱前衍生试剂。 溴乙烷被用做研究牛心脏线粒体中对寡霉素敏感的ATP酶的荧光标记。

特点和优势

用溴乙烷分析低分子量硫醇的好处在于： 高选择性。
  • 反应快速。
  • 易于通过反相HPLC分离衍生物。
  • 能穿透细胞。

包装

无底玻璃瓶。内含物在插入的融合锥体内。

其他说明

荧光硫醇特异性试剂

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储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

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分析证书(COA)

Lot/Batch Number
BCCM0976
BCCB2263
BCCB4375
BCCB4376
BCBF5306V

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P C Chinn et al.
Analytical biochemistry, 159(1), 143-149 (1986-11-15)
A highly sensitive and specific assay for Escherichia coli thioredoxin was developed using the thiol-specific reagent monobromobimane. Treatment of dithiothreitol-reduced thioredoxin with an excess of monobromobimane in Tris buffer (pH 8.0, 23 degrees C) for 30 min resulted in the
R M Maiorino et al.
Journal of chromatography, 374(2), 297-310 (1986-01-24)
The increasing therapeutic use of dithiol metal binding agents, such as 2,3-dimercaptopropane-1-sulfonic acid (DMPS), has stimulated the need for a sensitive and selective method for their determination in biological fluids. A method has now been developed in which DMPS was
Florencia Sardi et al.
Analytical biochemistry, 435(1), 74-82 (2013-01-09)
A method based on the differential reactivity of thiol and thiolate with monobromobimane (mBBr) has been developed to measure nucleophilicity and acidity of protein and low-molecular-weight thiols. Nucleophilicity of the thiolate is measured as the pH-independent second-order rate constant of
Michael Hall et al.
Proteomics, 10(5), 987-1001 (2010-01-06)
The light-dependent regulation of stromal enzymes by thioredoxin (Trx)-catalysed disulphide/dithiol exchange is known as a classical mechanism for control of chloroplast metabolism. Recent proteome studies show that Trx targets are present not only in the stroma but in all chloroplast
Carl Simonsson et al.
The Journal of investigative dermatology, 131(7), 1486-1493 (2011-01-14)
Allergic contact dermatitis (ACD) is the most prevalent form of human immunotoxicity. It is caused by skin exposure to haptens, i.e., protein-reactive, low-molecular-weight chemical compounds, which form hapten-protein complexes (HPCs) in the skin, triggering the immune system. These immunogenic HPCs
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