所有图片(1)
别名:
咪唑 溶液, 甘恶啉 溶液
经验公式（希尔记法）:
C3H4N2
CAS号:
分子量:
68.08
Beilstein:
103853
MDL编号:
UNSPSC代码:
12161700
PubChem化学物质编号:
NACRES:
NA.25
推荐产品
产品线
BioUltra
质量水平
形式
liquid
浓度
1 M in H2O
杂质
DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected
pH值（酸碱度）
9.5±0.5
密度
1.01 g/mL at 20 °C
SMILES字符串
c1c[nH]cn1
InChI
1S/C3H4N2/c1-2-5-3-4-1/h1-3H,(H,4,5)
InChI key
RAXXELZNTBOGNW-UHFFFAOYSA-N
一般描述
咪唑可用作在25°C下、6.2-7.8的pH范围内的缓冲液。推荐将其作为测定辣根过氧化物酶的缓冲液的组分和作为各种二价阳离子结合的螯合剂。咪唑可用于从二价阳离子树脂（Sigma P6611，HisSelect.-HC镍亲和凝胶）洗脱含组氨酸的蛋白质，也可用于SDS-PAGE凝胶的反向染色以检测蛋白质。
应用
咪唑缓冲溶液已用于一种组分制备：
- 钙调蛋白结合缓冲液1（CBB1），用于串联亲和纯化（TAP）TAP标记的蛋白质与共纯化蛋白质
- 裂解缓冲液，用于His-泛素下接实验
- 咪唑洗涤和洗脱缓冲液，用于蛋白质纯化
警示用语：
Danger
危险声明
危险分类
Eye Dam. 1 - Repr. 1B - Skin Corr. 1C
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
Peter Canning et al.
Journal of molecular biology, 426(13), 2457-2470 (2014-04-29)
The discoidin domain receptors (DDRs), DDR1 and DDR2, form a unique subfamily of receptor tyrosine kinases that are activated by the binding of triple-helical collagen. Excessive signaling by DDR1 and DDR2 has been linked to the progression of various human
Ali Tebbi et al.
The Journal of biological chemistry, 290(22), 14077-14090 (2015-04-17)
Ribonucleotide reductase (RnR) is a key enzyme synthesizing deoxyribonucleotides for DNA replication and repair. In mammals, the R1 catalytic subunit forms an active complex with either one of the two small subunits R2 and p53R2. Expression of R2 is S
Pam M Van Ry et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 23(8), 1285-1297 (2015-06-09)
Duchenne muscular dystrophy (DMD) is a fatal neuromuscular disease caused by mutations in the dystrophin gene, leading to the loss of a critical component of the sarcolemmal dystrophin glycoprotein complex. Galectin-1 is a small 14 kDa protein normally found in skeletal
Michael D Clark et al.
Proceedings of the National Academy of Sciences of the United States of America, 112(28), E3669-E3678 (2015-07-01)
Acetylation is correlated with chromatin decondensation and transcriptional activation, but its regulation by histone deacetylase (HDAC)-bearing corepressor complexes is poorly understood. Here, we describe the mechanism of assembly of the mammalian Sin3L/Rpd3L complex facilitated by Sds3, a conserved subunit deemed
Ranjana Pal et al.
The Journal of biological chemistry, 290(37), 22520-22531 (2015-07-30)
CUX1 and CUX2 proteins are characterized by the presence of three highly similar regions called Cut repeats 1, 2, and 3. Although CUX1 is ubiquitously expressed, CUX2 plays an important role in the specification of neuronal cells and continues to
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