BIONi010-C-15

Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.

The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.

Depositor
Bioneer

Derivation
Primary cell type: -
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Gene list:
KLF4
Lin28
MYC
POU5F1
shP53
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr

Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no

Characterization
Analysis of Undifferentiated Cells
Morphology:
1 day after thawing_1.tiff
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -

Genotyping
Karyotyping
Passage number: 27
Cell line karyotype: 46, XY
Karyotyping method: G-Banding
Image / Result

Genetic Modification
Modifications not associated with diseases
Type of modification: Transgene expression
Chromosome location: AAVS1 locus
Delivery method: TALEN
Description: Enforced NGN2-GFP expression triggers transdifferentiation of iPS cells to GFP positive neurons

Note: EAUA and CLIP must be completed before order fulfillment

Surface coating: Matrigel^®/Geltrex^®
Passage method: Enzyme-free cell dissociation / EDTA
O₂ concentration: 18%
CO₂ concentration: 5%
Culture medium: Essential E8^®
Passage Number banked (pre-EBiSC): 28
No. Vials banked (pre-EBiSC): 30

E8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation

EBiSC is a trademark of Fraunhofer-Gesellschaft

GELTREX is a registered trademark of Life Technologies Corporation

Matrigel is a registered trademark of Corning, Inc.