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64958

2-甲氧基-2,4-二苯基-3(2H)-呋喃酮

Name: 2-甲氧基-2,4-二苯基-3(2<I>H</I>)-呋喃酮
Brand: SIGMA

suitable for fluorescence, ≥98.0% (HPLC)

别名:

2,4-Diphenyl-2-methoxy-3(2H)-furanone, MDPF

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About This Item

经验公式（希尔记法）:

C₁₇H₁₄O₃

CAS号:

50632-57-0

分子量:

266.29

Beilstein:

1288529

MDL编号:

MFCD00036834

UNSPSC代码:

12352108

PubChem化学物质编号:

329760016

NACRES:

NA.32

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此商品	Z677728	Z677736	Z677701
Z677698 Vitoject ^™ 氟碳橡胶手套	Z677728 Vitoject ^™ 氟碳橡胶手套	Z677736 Vitoject ^™ 氟碳橡胶手套	Z677701 Vitoject ^™ 氟碳橡胶手套
manufacturer/tradename KCL 890	manufacturer/tradename KCL 890	manufacturer/tradename KCL 890	manufacturer/tradename KCL 890
material black (fluorocarbon rubber), black gloves	material black (fluorocarbon rubber), black gloves	material black (fluorocarbon rubber), black gloves	material black (fluorocarbon rubber), black gloves
packaging pack of 1 pair	packaging pack of 1 pair	packaging pack of 1 pair	packaging pack of 1 pair
size S (8)	size L (10)	size XL (11)	size M (9)
sterility non-sterile	sterility non-sterile	sterility non-sterile	sterility non-sterile

应用

2-Methoxy-2,4-diphenyl-3(2H)-furanone (MDPF) is used as a fluorescence reagent to derivatize primary and secondary amines on molecules such as proteins. MDPF derivatized molecules can be detected during analytical and separation procedures including chromatography, electrophoresis and zymography.

其他说明

Reagent for the derivatization of primary and secondary amines for HPLC; highly fluorescent derivatives are formed[1][2]; Pre-column derivatization of amines[3]; Fluorescent labeling of proteins before SDS-PAGE[4]

象形图

GHS07

警示用语：

Warning

危险声明

H315,H319,H335

预防措施声明

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

危险分类

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Gloves

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Macromolecular transport across endothelial monolayers.

R C Schaeffer et al.

International journal of microcirculation, clinical and experimental, 11(2), 181-201 (1992-05-11)

We studied the macromolecular size-selective transport characteristics of polycarbonate (PC) filters with defined pore radius (rp; 15,000 to 400 A) as well as 2,000 A rp PC filter-bovine pulmonary artery endothelial cell (EC) monolayer sandwich under zero hydrostatic pressure conditions

Rapid fluorescent monitoring of total protein patterns on sodium dodecyl sulfate-polyacrylamide gels and western blots before immunodetection and sequencing.

F J Alba et al.

Electrophoresis, 19(14), 2407-2411 (1998-11-20)

The fluorogenic dye 2-methoxy-2,4-diphenyl-3(2H)-furanone (MDPF) has been used for the detection of total protein patterns on polyvinylidene difluoride (PVDF) membranes. Fluorescent staining of protein bands on membranes with this covalent dye is completed in 20 min. Wet membranes are translucent

Endocytosis and exocytosis of protein in capillary endothelium.

S K Williams et al.

Journal of cellular physiology, 120(2), 157-162 (1984-08-01)

The transport of proteins across continuous capillary endothelium is believed to be mediated by micropinocytic vesicles which shuttle molecules between the lumenal and abluminal plasma membrane. We have studied the ability of capillary endothelial cells isolated from rat epididymal fat

2-Methoxy-2,4-diphenyl-3(2H)-furanone-labeled gelatin zymography and reverse zymography: a rapid real-time method for quantification of matrix metalloproteinases-2 and -9 and tissue inhibitors of metalloproteinases.

Danqing Min et al.

Electrophoresis, 27(2), 357-364 (2005-12-07)

Measurement of matrix metalloproteinases (MMPs) and their specific tissue inhibitors of metalloproteinases (TIMPs) by the techniques of zymography and reverse zymography provide useful information regarding the status of matrix accumulation or breakdown. This report describes the use of 2-methoxy-2,4-diphenyl-3(2H)-furanone (MDPF)

Rapid imaging, using a cooled charge-coupled-device, of fluorescent two-dimensional polyacrylamide gels produced by labelling proteins in the first-dimensional isoelectric focusing gel with the fluorophore 2-methoxy-2,4-diphenyl-3(2H)furanone.

P Jackson et al.

Electrophoresis, 9(7), 330-339 (1988-07-01)

A new method for visualising proteins in two-dimensional polyacrylamide gels was developed. Proteins were labelled with the fluorophore 2-methoxy-2,4-diphenyl-3(2H)furanone (MDPF) while present in the first-dimensional gel after isoelectric focusing and subsequently electrophoresed into the second-dimensional gel. High resolution spot patterns

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2-甲氧基-2,4-二苯基-3(2H)-呋喃酮

suitable for fluorescence, ≥98.0% (HPLC)

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WGK

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