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Merck
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安全信息

64958

Sigma-Aldrich

2-甲氧基-2,4-二苯基-3(2H)-呋喃酮

suitable for fluorescence, ≥98.0% (HPLC)

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别名:
2,4-Diphenyl-2-methoxy-3(2H)-furanone, MDPF
经验公式（希尔记法）:
C17H14O3
CAS号:
50632-57-0
分子量:
266.29
Beilstein:
1288529
MDL编号:
MFCD00036834
UNSPSC代码:
12352200
PubChem化学物质编号:
329760016
NACRES:
NA.32

质量水平

100

检测方案

≥98.0% (HPLC)

形式

solid

荧光

λex 384 nm; λem 472 nm in acetonitrile (after derivatization with hexylamine)

适用性

suitable for fluorescence

储存温度

2-8°C

SMILES字符串

COC1(OC=C(C1=O)c2ccccc2)c3ccccc3

InChI

1S/C17H14O3/c1-19-17(14-10-6-3-7-11-14)16(18)15(12-20-17)13-8-4-2-5-9-13/h2-12H,1H3

InChI key

BLWINLJDTOJSRU-UHFFFAOYSA-N

应用

2-Methoxy-2,4-diphenyl-3(2H)-furanone (MDPF) is used as a fluorescence reagent to derivatize primary and secondary amines on molecules such as proteins. MDPF derivatized molecules can be detected during analytical and separation procedures including chromatography, electrophoresis and zymography.

其他说明

Reagent for the derivatization of primary and secondary amines for HPLC; highly fluorescent derivatives are formed; Pre-column derivatization of amines; Fluorescent labeling of proteins before SDS-PAGE

象形图

Exclamation mark
GHS07

警示用语：

Warning

危险声明

H315,H319,H335

危险分类

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Gloves

法规信息

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V E Urwin et al.
Analytical biochemistry, 195(1), 30-37 (1991-05-15)
A multiple mini two-dimensional electrophoretic method which results in three two-dimensional protein spot patterns being positioned side by side in an individual gel has been developed. Preparation time has been minimized by employing disposable capillary tubes for the isoelectric focusing
H. Nakamura et al.
Analytical Chemistry, 56, 919-919 (1984)
Karen Martin et al.
Proteomics, 3(7), 1215-1227 (2003-07-23)
Three-color fluorescence detection methods are described based upon covalently coupling the dye 2-methoxy-2,4-diphenyl-2(2H)-furanone (MDPF) to proteins immobilized on poly(vinylidene difluoride) (PVDF) membranes, followed by detection of target proteins using alkaline-phosphatase-conjugated reporter molecules in combination with the fluorogenic substrate 9H-(1,3-dichloro-9,9-dimethylacridin-2-one-7-yl) phosphate
V Toome et al.
Biochemical and biophysical research communications, 114(2), 433-439 (1983-07-29)
2-Methoxy-2,4-diphenyl-3(2H)-furanone (MDPF) reacts readily with the free amino group of a dipeptide to form a pyrrolinone-type chromophore with absorption maxima at 275-285 and 370-390 nm. A simple test tube procedure is described which allows in situ correlation of the absolute
S. Stein
Pept. Neurobiol., 9-9 (1977)
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