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杂质
≥20% N,N-dimethyl-p-toluidine
密度
1.050 g/cm3
应用
hematology
histology
储存温度
room temp
应用
LR Accelerator is used for the chemical curing of LR White and LR Gold embedding media for routine and low temperature electron microscopy or the immunocytochemical localization of antigens by either light or electron microscopy.
警示用语:
Danger
危险分类
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 3 - Carc. 1B - Repr. 2 - Skin Sens. 1 - STOT RE 2 Oral
靶器官
Reproductive organs
储存分类代码
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 3
闪点(°F)
168.8 °F
闪点(°C)
76 °C
个人防护装备
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
Histochemistry and cell biology, 135(1), 103-110 (2010-12-15)
The best available approach of biological sample preparation for transmission electron microscopy currently includes cryoimmobilization by high-pressure freezing (HPF) followed by freeze-substitution (FS). This method has been well established for interphase cells; however, a reliable and easy procedure is still
Histochemistry and cell biology, 130(5), 1047-1052 (2008-09-18)
A protocol for high-pressure freezing and LR White embedding of mammalian cells suitable for fine ultrastructural studies in combination with immunogold labelling is presented. HeLa S3 cells enclosed in low-temperature gelling agarose were high-pressure frozen, freeze-substituted in acetone, and embedded
Acta histochemica, 110(5), 427-431 (2008-01-12)
Hydrophilic resins present the advantage of making possible both hybridization experiments involving either antibodies or oligonucleotide probes and ultrastructural observations. Whereas various embedding protocols are available, only very few concern flat-embedded preparations. In this study we describe an easy protocol
Histochemistry and cell biology, 134(6), 631-641 (2010-11-11)
In this study we present an optimized method of high-pressure freezing and automated freeze-substitution of cultured human cells, followed by LR White embedding, for subsequent immunolabeling. Also, the influence of various conditions of the freeze-substitution procedures such as temperature, duration
Methods in molecular biology (Clifton, N.J.), 657, 237-248 (2010-07-06)
We describe a standardized method of fixation, antigen retrieval, and image contrasting for post-embedding immunoelectron microscopy. Tissues are fixed with formaldehyde solutions containing Ca(2+) and Mg(2+) ions at pH 7.4 and then at pH 8.5. After dehydration with dimethylformamide, the
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