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Merck
CN
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主要文件

62660

Sigma-Aldrich

LR Accelerator

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About This Item

MDL编号:
UNSPSC代码:
12171500
NACRES:
NA.47

杂质

≥20% N,N-dimethyl-p-toluidine

密度

1.050 g/cm3

应用

hematology
histology

储存温度

room temp

应用

LR Accelerator is used for the chemical curing of LR White and LR Gold embedding media for routine and low temperature electron microscopy or the immunocytochemical localization of antigens by either light or electron microscopy.

相关产品

象形图

Skull and crossbonesHealth hazard

警示用语:

Danger

危险分类

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 3 - Carc. 1B - Repr. 2 - Skin Sens. 1 - STOT RE 2 Oral

靶器官

Reproductive organs

储存分类代码

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

闪点(°F)

168.8 °F

闪点(°C)

76 °C

个人防护装备

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


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Margarita Sobol et al.
Histochemistry and cell biology, 135(1), 103-110 (2010-12-15)
The best available approach of biological sample preparation for transmission electron microscopy currently includes cryoimmobilization by high-pressure freezing (HPF) followed by freeze-substitution (FS). This method has been well established for interphase cells; however, a reliable and easy procedure is still
Vendula Strádalová et al.
Histochemistry and cell biology, 130(5), 1047-1052 (2008-09-18)
A protocol for high-pressure freezing and LR White embedding of mammalian cells suitable for fine ultrastructural studies in combination with immunogold labelling is presented. HeLa S3 cells enclosed in low-temperature gelling agarose were high-pressure frozen, freeze-substituted in acetone, and embedded
Olivier Gros et al.
Acta histochemica, 110(5), 427-431 (2008-01-12)
Hydrophilic resins present the advantage of making possible both hybridization experiments involving either antibodies or oligonucleotide probes and ultrastructural observations. Whereas various embedding protocols are available, only very few concern flat-embedded preparations. In this study we describe an easy protocol
Margarita Sobol et al.
Histochemistry and cell biology, 134(6), 631-641 (2010-11-11)
In this study we present an optimized method of high-pressure freezing and automated freeze-substitution of cultured human cells, followed by LR White embedding, for subsequent immunolabeling. Also, the influence of various conditions of the freeze-substitution procedures such as temperature, duration
Shuji Yamashita
Methods in molecular biology (Clifton, N.J.), 657, 237-248 (2010-07-06)
We describe a standardized method of fixation, antigen retrieval, and image contrasting for post-embedding immunoelectron microscopy. Tissues are fixed with formaldehyde solutions containing Ca(2+) and Mg(2+) ions at pH 7.4 and then at pH 8.5. After dehydration with dimethylformamide, the

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