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表单
powder (fine)
质量水平
比活
~10 U/mg
分子量
Mr ~43000
储存温度
2-8°C
InChI
1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
InChI key
QWZUIMCIEOCSJF-CHHCPSLASA-N
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一般描述
米根霉脂肪酶（ROL）由一个氧阴离子洞、四个 N-糖基化位点和一个活性位点组成。它具有 N-端前序列和前序列。
Research Area: Cell Signaling
Rhizopus oryzae lipase (ROL) is a protein synthesized in a precursor form that includes a presequence of 26 amino acids, followed by a prosequence of 97 amino acids, which is attached to the N-terminal of a mature sequence consisting of 269 amino acids.
Rhizopus oryzae lipase (ROL) is a protein synthesized in a precursor form that includes a presequence of 26 amino acids, followed by a prosequence of 97 amino acids, which is attached to the N-terminal of a mature sequence consisting of 269 amino acids.
应用
使用米根霉脂肪酶：
- 测试其对 1,2-甘油二油酸酯合成和甘油三油酸酯乙醇分解的影响
- 固定氧化石墨烯载体，以便于生物催化研究
- 从莱茵衣藻和酿酒酵母中消化甘油三酯（TAG）
脂肪酶在工业上用于拆分手性化合物和生物柴油的酯交换生产。
生化/生理作用
米根霉脂肪酶（ROL）作为一种催化剂，用于通过水解逆转酶促合成聚甘油蓖麻醇酯。基于其 1,3-区域特殊性功能，ROL 在结构化脂质的工业生产中十分有用。
甘油三酸酯、甘油二酸酯和甘油单酸酯被水解（按降序排列）。
脂肪酶可催化三酰基甘油水解成甘油和游离的脂肪酸。
脂肪酶可催化三酰基甘油水解成甘油和游离的脂肪酸。
Rhizopus oryzae lipase (ROL) has been extensively researched for its regiospecificity in biodiesel production. Due to its remarkable characteristics, including 1,3-specificity, high enantioselectivity, and stability in organic solvents, ROL has garnered significant attention for applications in the energy, food, and pharmaceutical industries.
单位定义
1 U 相当于 pH 8.0，40°C 下每分钟释放 1 μmol 丁酸的酶量（三丁酸甘油酯，货号 91010，作为底物）如上所述，5000 U 相当于 ~1 U，使用三油酰甘油酯，货号 62314，作为底物，在 pH 8.0，40°C 下
其他说明
说明：以三乙酰甘油酯作为底物时，pH 为 7.4。孵化期：60 分钟。
催化油和脂肪的酯交换反应；用于去除电免疫测定载脂蛋白 B 中的干扰甘油三酯；通过对映选择性酶水解拆分外消旋环氧酯
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
T. Kim et al.
Enzyme and Microbial Technology, 11, 528-528 (1989)
Electroimmunoassay of apolipoprotein B in triacylglycerol-rich serum.
P Laburre et al.
Clinical chemistry, 31(5), 787-787 (1985-05-01)
Effective and highly selective lipase-mediated synthesis of 2-monoolein and 1, 2-diolein in a two-phase system
Serrano-Arnaldos JM, et al.
Journal of Molecular Catalysis. B, Enzymatic, 112, 9-14 (2014)
Rhizopus oryzae Lipase, a Promising Industrial Enzyme: Biochemical Characteristics, Production and Biocatalytic Applications
Lopez-Fernandez J, et al.
Catalysts, 10, 1277-1277 (2020)
Lipase enzymes on graphene oxide support for high-efficiency biocatalysis
HermanovaS, et al.
Applied Materials Today, 5 (2016)
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