一般描述
米根霉脂肪酶（ROL）由一个氧阴离子洞、四个 N-糖基化位点和一个活性位点组成。它具有 N-端前序列和前序列。
Research Area: Cell Signaling
Rhizopus oryzae lipase (ROL) is a protein synthesized in a precursor form that includes a presequence of 26 amino acids, followed by a prosequence of 97 amino acids, which is attached to the N-terminal of a mature sequence consisting of 269 amino acids.
Rhizopus oryzae lipase (ROL) is a protein synthesized in a precursor form that includes a presequence of 26 amino acids, followed by a prosequence of 97 amino acids, which is attached to the N-terminal of a mature sequence consisting of 269 amino acids.
应用
使用米根霉脂肪酶：
- 测试其对 1,2-甘油二油酸酯合成和甘油三油酸酯乙醇分解的影响
- 固定氧化石墨烯载体，以便于生物催化研究
- 从莱茵衣藻和酿酒酵母中消化甘油三酯（TAG）
脂肪酶在工业上用于拆分手性化合物和生物柴油的酯交换生产。
生化/生理作用
米根霉脂肪酶（ROL）作为一种催化剂，用于通过水解逆转酶促合成聚甘油蓖麻醇酯。基于其 1,3-区域特殊性功能，ROL 在结构化脂质的工业生产中十分有用。
Rhizopus oryzae lipase (ROL) has been extensively researched for its regiospecificity in biodiesel production. Due to its remarkable characteristics, including 1,3-specificity, high enantioselectivity, and stability in organic solvents, ROL has garnered significant attention for applications in the energy, food, and pharmaceutical industries.
甘油三酸酯、甘油二酸酯和甘油单酸酯被水解（按降序排列）。
脂肪酶可催化三酰基甘油水解成甘油和游离的脂肪酸。
脂肪酶可催化三酰基甘油水解成甘油和游离的脂肪酸。
单位定义
1 U 相当于 pH 8.0，40°C 下每分钟释放 1 μmol 丁酸的酶量（三丁酸甘油酯，货号 91010，作为底物）如上所述，5000 U 相当于 ~1 U，使用三油酰甘油酯，货号 62314，作为底物，在 pH 8.0，40°C 下
其他说明
说明：以三乙酰甘油酯作为底物时，pH 为 7.4。孵化期：60 分钟。
催化油和脂肪的酯交换反应；用于去除电免疫测定载脂蛋白 B 中的干扰甘油三酯；通过对映选择性酶水解拆分外消旋环氧酯
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
Rhizopus oryzae lipase, a promising industrial enzyme: Biochemical characteristics, production and biocatalytic applications
L'opez-Fern'andez J, et al.
Catalysts (Basel, Switzerland), 1277-1277 (2020)
Yeongho Kim et al.
Proceedings of the National Academy of Sciences of the United States of America, 115(7), 1652-1657 (2018-02-01)
Understanding the unique features of triacylglycerol (TAG) metabolism in microalgae may be necessary to realize the full potential of these organisms for biofuel and biomaterial production. In the unicellular green alga Chlamydomonas reinhardtii a chloroplastic (prokaryotic) pathway has been proposed
T. Kim et al.
Enzyme and Microbial Technology, 11, 528-528 (1989)
Effective and highly selective lipase-mediated synthesis of 2-monoolein and 1, 2-diolein in a two-phase system
Serrano-Arnaldos JM, et al.
Journal of Molecular Catalysis. B, Enzymatic, 112, 9-14 (2014)
Lipase enzymes on graphene oxide support for high-efficiency biocatalysis
HermanovaS, et al.
Applied Materials Today, 5 (2016)
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