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62288

Sigma-Aldrich

重组脂肪酶 B 南极假丝酵母 来源于米曲霉

powder, beige, ~9 U/mg

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About This Item

CAS号:
9001-62-1
EC 号:
3.1.1.3 (BRENDA, IUBMB)
EC 号:
232-619-9
MDL编号:
MFCD00131509
UNSPSC代码:
12352204
NACRES:
NA.54

重组

expressed in Aspergillus oryzae

质量水平

100

表单

powder

比活

~9 U/mg

分子量

33 kDa

颜色

beige

储存温度

2-8°C

InChI

1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;

InChI key

QWZUIMCIEOCSJF-CHHCPSLASA-N

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一般描述

南极假丝酵母脂肪酶b(CalB)结构上类似于其他几种脂酶，具有灵活的盖子结构。它由317个氨基酸构成，分子量为33kDa。脂肪酶b属于α/β折叠水解酶家族成员。

应用

米曲霉重组生产的南极假丝酵母脂肪酶b已用于：
  • 作为D5-CalB酶特性鉴定的标准品
  • 作为高效的生物催化剂催化外消旋仲醇的酯化反应获得(R)-酯
  • 研究基于反应挤出的聚丁二酸丁二醇酯的“绿色”再生途径
  • 比较吸附CaLB (aCaLB)和共价固定化CaLB (cCaLB)的酯化产率

脂肪酶在工业上用于手性化合物的拆分和生物柴油的酯交换生产。

生化/生理作用

来自南极假丝酵母的脂肪酶 B 已证明是一个有效的催化剂，用于从大于辛酸的脂肪酸合成乙基 D -吡喃葡萄糖苷的酯。它也被发现催化各种各样的有机反应，包括许多不同的区域和对映选择性合成。
脂肪酶催化三酰基甘油水解为甘油和游离脂肪酸。
南极假丝酵母脂肪酶b(CALB)具有广泛的底物专一性、高活性和高立体选择性，因此被视为重要的生物技术用酶。它还可在水溶性和非水溶性两种反应条件下发挥作用。CALB可用于酯交换、动力学拆分和聚合反应。

单位定义

1 U 相当于 pH 8.0，40°C 下每分钟释放 1 μmol 丁酸的酶量（三丁酸甘油酯作为底物，产品目录号：91010）

象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H334

预防措施声明

P261 - P284 - P501

危险分类

Resp. Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
BCCL7188
BCCG5108
BCBP3380V
BCBW4983

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What solvent can Lipase B Candida antarctica, recombinant from Aspergillus oryzae, Product 62288, be dissolved in?

    Lipase B is expected to be soluble in water. We are able to dissolve the enzyme in water for the enzymatic assay. We also have tested the solubility of Lipase B in 0.05M potassium phosphate buffer solution (pH=7.5) during quality control at a concentration of 10mg/mL. According to the chemicals encyclopedia published by the Royal Society of Chemistry, almost all organic solvents decrease lipase activity, with the exception of petroleum ether.  

  4. Is Product 62288, Lipase B Candida antarctica, recombinant from Aspergillus oryzae, lyophilized?

    This lipase is a lyophilisate and may contain Gum arabic for adjustment of activity. The amount of Gum arabic in each vial is lot dependent.

  5. How pure is Product 62288, Lipase B Candida antarctica, recombinant from Aspergillus oryzae?

    Purity is not determined for this product. As is the case with many of our enzymes, the product is assayed for enzymatic activity, not for purity.

  6. What is the molecular weight of Lipase B Candida antarctica, recombinant from Aspergillus oryzae, Product 62288?

    Unfortunately, molecular weight information is unavailable at this time.

  7. What is Product 62288, Lipase B Candida antarctica, recombinant from Aspergillus oryzae, soluble in?

    Lipase B is expected to be soluble in water. We are able to dissolve the enzyme in water for the enzymatic assay. We also have tested the solubility of Lipase B in 0.05 M potassium phosphate buffer solution (pH=7.5) during quality control at a concentration of 10 mg/mL. According to the chemicals encyclopedia published by the Royal Society of Chemistry, almost all organic solvents decrease lipase activity, with the exception of petroleum ether.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Preparation of passion fruit-typical 2-alkyl ester enantiomers via lipase-catalyzed kinetic resolution
Strohalm H, et al.
Journal of Agricultural and Food Chemistry, 58(10), 6328-6333 (2010)
Sol Cuenca et al.
Frontiers in plant science, 7, 464-464 (2016-05-06)
Elongated flexuous plant viral nanoparticles (VNPs) represent an interesting platform for developing different applications in nanobiotechnology. In the case of potyviruses, the virion external surface is made up of helically arrayed domains of the viral structural coat protein (CP), repeated
A novel self-activation mechanism of Candida antarctica lipase B
Luan B and Zhou R
Physical Chemistry Chemical Physics, 19(24), 15709-15714 (2017)
A green method for polybutylene succinate recycling: Depolymerization catalyzed by lipase B from Candida antarctica during reactive extrusion
Jbilou F, et al.
European Polymer Journal, 68, 207-215 (2015)
Wenjie Yang et al.
JACS Au, 1(12), 2172-2181 (2022-01-04)
The immobilization of enzymes in metal-organic frameworks (MOFs) with preserved biofunctionality paves a promising way to solve problems regarding the stability and reusability of enzymes. However, the rational design of MOF-based biocomposites remains a considerable challenge as very little is
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