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等级
for microscopy (Bot., Hist.)
质量水平
表单
powder
技术
microbe id | staining: suitable
溶解性
methanol: water (1:1): 0.01 g/10 mL, red
εmax
≥275 at 550-560 nm in DMSO
≥300 at 516-526 nm in DMSO
应用
diagnostic assay manufacturing
hematology
histology
储存温度
room temp
SMILES字符串
[Na+].Nc1c(O)c(c(O)c2C(=O)c3ccccc3C(=O)c12)S([O-])(=O)=O
InChI
1S/C14H9NO7S.Na/c15-9-7-8(12(18)14(13(9)19)23(20,21)22)11(17)6-4-2-1-3-5(6)10(7)16;/h1-4,18-19H,15H2,(H,20,21,22);/q;+1/p-1
InChI key
IFSXZLJQEKGQAF-UHFFFAOYSA-M
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一般描述
核固红（C.l.60760）是一种取代的羟基蒽醌发色团，可以螯合金属离子。 它也被称为 kernechtrot 和 4-氨基-9,10-二氢-1,3-二羟基-9,10-双氧-2-蒽磺酸钠盐。可用于人类医学细胞诊断，并用于人类来源样本材料的组织学研究。它是一种干染料，适合人组织标本（例如，肾脏组织切片）诊断用制片流程中的染色步骤。【制片流程：固定、包埋（需要时）、核固红溶液染色、复染、封片。】
应用
核固红已被用于鸽子嘴侧表皮和上喙冠状区域的PB（普鲁士蓝）阳性细胞的核染色。最近的应用包括：
- 精液中精子和精子细胞的染色
- 石蜡包埋组织中用于 PCR 分析的区域的鉴定
- 免疫染色和银染色后的细胞核复染色
用作生物染色剂
生化/生理作用
核固红染料具有蒽醌性质，可单独使用或与其他染料组合使用。它主要用于细胞核染色。
原理
核固红可用于简单的细胞核染色。它是一种蒽醌类酸性染料。可添加硫酸铝作为媒染剂，形成色淀，促使细胞核染成红色。核固红-硫酸铝用于组织化学反应中的复染，例如用于检测铁的柏林蓝染色或用于银染。核固红（Certistain®，显微镜用）也可与阿尔新蓝共同用于核染色。
法律信息
CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
dust mask type N95 (US), Eyeshields, Gloves
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