推荐产品
产品名称
氯酚红-β-D-吡喃半乳糖苷, ≥90% (HPLC)
方案
≥90% (HPLC)
表单
powder or crystals
溶解性
water: 20 mg/mL, clear, orange to very deep red
储存温度
−20°C
SMILES字符串
OC[C@H]1O[C@@H](Oc2ccc(cc2Cl)C(=C3/C=CC(=O)C(Cl)=C3)\c4ccccc4S(O)(=O)=O)[C@H](O)[C@@H](O)[C@H]1O
InChI
1S/C25H22Cl2O10S/c26-15-9-12(5-7-17(15)29)21(14-3-1-2-4-20(14)38(33,34)35)13-6-8-18(16(27)10-13)36-25-24(32)23(31)22(30)19(11-28)37-25/h1-10,19,22-25,28,30-32H,11H2,(H,33,34,35)/b21-12-/t19-,22+,23+,24-,25-/m1/s1
InChI key
YOUWVNCQJOMMEU-AETRGLENSA-N
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一般描述
氯酚红-β-D-吡喃半乳糖苷(CPRG)是一种长波长黄色染料，常用于比色检测。它可作为 β-半乳糖苷酶 (β-gal) 的底物，半乳糖苷酶可水解CPRG 形成氯酚红（紫色）。该产品通过测量其在 570 nm 处的吸光度进行定量检测。CPRG 用于鉴定粪便大肠菌群。它的灵敏度比邻-硝基苯基-β-半乳糖苷（ONPG）等显色剂高10倍左右。CPRG还具有很高的快速转化率。
应用
氯酚红-β-D-吡喃半乳糖苷已被用作 β-半乳糖苷(β-ga）的底物：
- 用于靶RNA检测的基于纸的物细胞蛋白质表达体系
- 采用lacZ输出基因进行多个临床相关突变的比色检测
- 用于细菌定量的比色测定
其他说明
测量转染细胞中 β-半乳糖苷酶的表达水平。lacZ 基因是一种在转染反应中常用的报告基因，因为它的产物 β-半乳糖苷酶是一种非常稳定的酶。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
监管及禁止进口产品
C Vega et al.
Parasitology research, 95(4), 296-298 (2005-02-01)
We have developed a new pharmacological screening assay for epimastigotes of Trypanosoma cruzi (clone CL-B5) that express the Escherichia coli LacZ gene. The assay is based on determining the activity of the cytoplasmic beta-galactosidase released into the culture on membrane
D C Eustice et al.
BioTechniques, 11(6), 739-740 (1991-12-01)
A sensitive method has been developed for the detection of E. coli beta-galactosidase in transfected HeLa cells. The chromogenic substrate, CPRG (chlorophenol red-beta-D-galactopyranoside), was compared with ONPG (o-nitrophenyl-beta-D-galactopyranoside) by kinetic analysis with purified beta-galactosidase. The Km for CPRG was 1.35
V C Wutor et al.
Chemosphere, 68(4), 622-627 (2007-04-17)
The presence of coliforms in polluted water was determined enzymatically (in situ) by directly monitoring the activity of beta-d-galactosidase (B-GAL) through the hydrolysis of the yellow chromogenic subtrate, chlorophenol red beta-d-galactopyranoside (CPRG), which produced a red chlorophenol red (CPR) product.
Generation of antigen-specific, lacZ-inducible T-cell hybrids.
S Malarkannan et al.
Methods in molecular biology (Clifton, N.J.), 156, 265-272 (2000-11-09)
Monpichar Srisa-Art et al.
Analytical chemistry, 90(1), 1035-1043 (2017-12-07)
Salmonella causes over a million foodborne illnesses per year in the United States resulting in more hospitalizations and deaths than any other foodborne bacterial pathogen. To help prevent outbreaks, a rapid, portable, sensitive, and reliable method for onsite detection of
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