所有图片(1)
别名:
1,5-I-AEDANS, 5-[2-(碘乙酰氨基)乙氨基]萘-1-磺酸, N-碘乙酰基-N′-(5-磺基-1-萘基)乙二胺, Hudson-Weber 试剂
线性分子式:
ICH2CONHCH2CH2NHC10H6SO3H
CAS号:
分子量:
434.25
Beilstein:
3004454
EC 号:
MDL编号:
UNSPSC代码:
12352200
eCl@ss:
32035301
PubChem化学物质编号:
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检测方案
≥95% (HPLC)
荧光
λex 336 nm; λem 490 nm (pH 8.0)
λex 337 nm; λem 498 nm (after derivatization with 2-mercaptoethanol)
适用性
suitable for fluorescence
储存温度
2-8°C
SMILES字符串
OS(=O)(=O)c1cccc2c(NCCNC(=O)CI)cccc12
InChI
1S/C14H15IN2O4S/c15-9-14(18)17-8-7-16-12-5-1-4-11-10(12)3-2-6-13(11)22(19,20)21/h1-6,16H,7-9H2,(H,17,18)(H,19,20,21)
InChI key
ZMERMCRYYFRELX-UHFFFAOYSA-N
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应用
N-(Iodoacetaminoethyl)-1-naphthylamine-5-sulfonic acid (1,5-I-AEDANS) is a fluorescence probe used to label protein sulfhydryls to study conformational changes, fluorescence anisotropy and protein:protein interactions.
其他说明
Fluorescent sulfhydryl reagent
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
Anjali Jha et al.
Journal of molecular biology, 393(3), 735-752 (2009-09-01)
The aggregation of proteins often results in highly ordered fibrillar structures. While significant insights have been obtained on structural aspects of amyloid fibrils, little is known about the structures of protofibrils, which are presumed to be the precursors of fibrils.
Zofia Ostrowska-Podhorodecka et al.
Archives of biochemistry and biophysics, 682, 108280-108280 (2020-01-31)
Tropomyosin and cofilin are involved in the regulation of actin filament dynamic polymerization and depolymerization. Binding of cofilin changes actin filaments structure, leading to their severing and depolymerization. Non-muscle tropomyosin isoforms were shown before to differentially regulate the activity of
Joseph J Kehoe et al.
Journal of agricultural and food chemistry, 55(17), 7107-7113 (2007-07-26)
This paper takes a new approach to determining which sulfhydryl groups are exposed during the heat denaturation of bovine beta-lactoglobulin A. The sulfhydryl groups exposed after heating were blocked with 5-((((2-iodoacetyl)amino)ethyl)amino)naphthalene-1-sulfonic acid (IAEDANS). The results show that IAEDANS is a
Martin Karlsson et al.
Biophysical journal, 88(5), 3536-3544 (2005-02-26)
Little is known about the direction and specificity of protein adsorption to solid surfaces, a knowledge that is of great importance in many biotechnological applications. To resolve the direction in which a protein with known structure and surface potentials binds
Thorsten Beitlich et al.
PloS one, 8(10), e78384-e78384 (2013-11-10)
The globular 25 kDa protein cytosine monophosphate kinase (CMPK, EC ID: 2.7.4.14) from E. coli belongs to the family of nucleoside monophosphate (NMP) kinases (NMPK). Many proteins of this family share medium to high sequence and high structure similarity including
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