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一般描述
浸镜油是一种透明、粘稠的液体,具有优化的折射率,经过专门改性,使其尽可能接近玻璃的折射率(RI) (ne = 1.5)。将它与物镜配合使用,可提高分辨率。
应用
浸泡油用于高分辨率(1000倍)光学显微镜工作,与油浸泡物镜结合使用,以在固定、嵌入、染色或反染色和安装后优化组织学、细胞学、血液学和细菌标本材料的显微检查。
原理
将浸镜油滴加到染色的已安装或未安装的试样材料上,在试样与显微镜透镜之间形成一层透明膜,以消除入射光的偏转,从而大大提高透镜的光学效率。
警示用语:
Warning
危险声明
预防措施声明
危险分类
Aquatic Acute 1 - Aquatic Chronic 2
储存分类代码
10 - Combustible liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
法规信息
监管及禁止进口产品
Theis Sommer et al.
Scientific reports, 8(1), 13104-13104 (2018-09-01)
The catalytic mechanism of the cyclic amidohydrolase isatin hydrolase depends on a catalytically active manganese in the substrate-binding pocket. The Mn2+ ion is bound by a motif also present in other metal dependent hydrolases like the bacterial kynurenine formamidase. The
Thomas P Burghardt et al.
Applied optics, 48(32), 6120-6131 (2009-11-12)
Total internal reflection fluorescence (TIRF) microscopy uses the evanescent field on the aqueous side of a glass/aqueous interface to selectively illuminate fluorophores within approximately 100 nm of the interface. Applications of the method include epi-illumination TIRF, where the exciting light
P L Appleton et al.
Journal of microscopy, 234(2), 196-204 (2009-04-29)
Visualizing overall tissue architecture in three dimensions is fundamental for validating and integrating biochemical, cell biological and visual data from less complex systems such as cultured cells. Here, we describe a method to generate high-resolution three-dimensional image data of intact
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