描述
for research or for further manufacturing use
质量水平
形式
powder
技术
cell culture | mammalian: suitable
组分
phenol red: yes
sodium pyruvate: yes
L-glutamine: yes
glucose: high
运输
ambient
储存温度
2-8°C
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应用
Dulbecco′s Modified Eagle′s Medium（DMEM）是对Basal Medium Eagle (BME)的一种改良，含有四倍浓度的氨基酸和维生素。 原始的配方含有1000 mg/L的葡萄糖并用于培养小鼠胚胎细胞。 从此，它被按照多种方式进行修改以支持小鼠和鸡细胞的原代培养，以及多种正常和转化细胞。 这些培养基都可提供一种不同的L-谷氨酰胺和丙酮酸钠组成。 此外，葡萄糖浓度也被提高至4500 mg/L，因此被命名为“DMEM/High”。
数量
按照每升培养基13.5克粉末配制。
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物来源培养基
Mengxi Jiang et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(26), 15055-15065 (2020-06-20)
Phosphocholine phosphatase-1 (PHOSPHO1) is a phosphocholine phosphatase that catalyzes the hydrolysis of phosphocholine (PC) to choline. Here we demonstrate that the PHOSPHO1 transcript is highly enriched in mature brown adipose tissue (BAT) and is further induced by cold and isoproterenol
Marko Knoll et al.
Nature communications, 8(1), 2115-2115 (2017-12-14)
Brown adipose tissue (BAT) metabolism influences glucose homeostasis and metabolic health in mice and humans. Sympathetic stimulation of β-adrenergic receptors in response to cold induces proliferation, differentiation, and UCP1 expression in pre-adipocytes and mature brown adipocytes. Here we show that
Maria Masnata et al.
Acta neuropathologica, 137(6), 981-1001 (2019-02-23)
In recent years, evidence has accumulated to suggest that mutant huntingtin protein (mHTT) can spread into healthy tissue in a prion-like fashion. This theory, however, remains controversial. To fully address this concept and to understand the possible consequences of mHTT spreading
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