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52412

Sigma-Aldrich

R -藻红蛋白

BioReagent, passes test for gel electrophoresis

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别名:
R-PE, 藻胆蛋白
CAS号:
MDL编号:
UNSPSC代码:
12352200
NACRES:
NA.32

产品线

BioReagent

质量水平

浓度

≥10.0 mg/mL (UV)

技术

electrophoresis: suitable

荧光

λex 542 nm; λem 575 nm in 0.1 M phosphate pH 7.2

适用性

passes test for gel electrophoresis

储存温度

2-8°C

相关类别

一般描述

R-藻红蛋白或R-PE是一种从大型藻类中获得的藻胆蛋白类荧光探针。 R-藻红蛋白(R-PE)是一种可用作 红藻 (红藻门)光合辅助色素的蛋白质。 它是一种240 kDa的寡聚蛋白,具有6个α(约20 kDa)、6个β(约20 kDa)和1个γ(约30 kDa)亚单位。 藻胆蛋白是一种具有高荧光特性的水溶性光捕获蛋白。

应用

藻红蛋白(R-PE),在实验室中可作为蓝藻存在和各种免疫荧光应用的基于荧光的指标。在各种电泳过程中,R-PE是一种有用的荧光探针。 R-藻红蛋白(R-PE)可检测土壤和地下水样品中的汞离子。 藻红蛋白已广泛应用于食品、化妆品、免疫诊断、分析试剂等领域。

分析说明

本品混悬于150 mM磷酸钠、60%硫酸铵、1 mM EDTA、1 mM 叠氮化钠(pH 7.0)中,偶联前必须用偶联缓冲液或PBS透析。A566/A280 >4.6、A620/A566 <0.03、A566/A498 <1.5

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves

法规信息

动植物源性产品

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R Rossano et al.
Journal of biotechnology, 101(3), 289-293 (2003-03-05)
R-Phycoerythrin (R-PE) is a protein acting as a photosynthetic accessory pigment in red algae (Rodophyta). This protein has gained importance in many biotechnological applications in food science, immunodiagnostic, therapy, cosmetics, protein and cell labelling, and analytical processes. In this paper
Xiaobin Wang et al.
The Analyst, 144(12), 3892-3897 (2019-05-24)
Mercury, as one of the most prevalent toxic metals released by various natural and anthropogenic processes, causes severe pollution of soil and groundwater. In this work, R-phycoerythrin (R-PE) proteins encapsulated into ZIF-8 composite thin films were prepared via a solid-confinement
Radek Sachl et al.
Biophysical journal, 101(11), L60-L62 (2012-01-21)
Even though superresolution microscopy indicates that size of plasma membrane rafts is <20 nm, those structures have never been observed. Förster resonance energy transfer (FRET) is therefore still the most powerful optical method for characterization of such domains. In this letter
Kun Tang et al.
Biochimica et biophysica acta, 1817(7), 1030-1036 (2012-04-03)
Phycobilisomes, the light-harvesting antennas in cyanobacteria and red algae, consist of an allophycocyanin core that is attached to the membrane via a core-membrane linker, and rods comprised of phycocyanin and often also phycoerythrin or phycoerythrocyanin. Phycobiliproteins show excellent energy transfer
David Rizzo et al.
Cytometry. Part B, Clinical cytometry, 84(2), 125-132 (2012-12-12)
Flow cytometry is the reference technique for assessing ZAP70 expression, a marker of poor prognosis in CLL. One of the most common methods is to assess ZAP70 levels in CLL cells by calculating the ratio between ZAP70 mean fluorescence intensities

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