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Merck
CN
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文件

安全信息

51411C

SAFC

最低必需培养基

with Earle′s Balanced Salts, with 2.0 mM L-glutamine, liquid, sterile-filtered, suitable for cell culture

Pharma Manufacturing

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别名:
Eagle最低必需培养基, MEM
UNSPSC代码:
12352207
NACRES:
NA.75

描述

for research or for further manufacturing use

质量水平

500

无菌性

sterile-filtered

形式

liquid

技术

cell culture | mammalian: suitable

组分

Earle’s salts (5% CO2): yes
phenol red: 10.62 mg/L
NaHCO3: 2200 mg/L
L-glutamine: 290 mg/L

运输

ambient

储存温度

2-8°C

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相关类别

一般描述

Harry Eagle研究了小鼠L细胞和HeLa细胞在培养中的基本营养需求，因此开发了含有Earle′s平衡盐的最低必需培养基（MEM）。 他证明这些细胞需要13种氨基酸和7种维生素才能在体外生长和繁殖。 MEM是Eagle′s早期培养基基础培养基Eagle（BME）的改良，含有更高浓度的必需营养素。 这些培养基已证明能够在培养中支持多种正常细胞和转化细胞，并含有Earle′s平衡盐，这使其适合在装有CO2的大气中使用。

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

动植物来源培养基

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